Robust salivary gland‐specific transgene expression in Anopheles stephensi mosquito
Robust salivary gland‐specific transgene expression in Anopheles stephensi mosquito
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DOI：
10.1111/j.1365-2583.2006.00645.x
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发表时间：
2006-08
影响因子：
2.6
通讯作者：
S. Yoshida;H. Watanabe
中科院分区：
文献类型：
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作者：
S. Yoshida;H. Watanabe
疟原虫子孢子侵入蚊子的唾液腺，并在唾液管中等待下一次吸血。唾液腺侵袭的过程和分子机制仍不清楚。为了在斯氏按蚊中建立稳健的唾液腺特异性转基因表达，我们获得了编码按蚊抗血小板蛋白（AAPP）的基因的唾液腺特异性启动子。aapp启动子是女性唾液腺特异性和血餐诱导型强启动子。利用该启动子，我们获得了转基因An。stephensi在腺体的远侧叶中表达丰富的Discosoma sp.红色荧光蛋白（DsRed），子孢子优先侵入。这些结果开辟了阐明唾液腺寄生虫相互作用和产生转基因蚊子难治寄生虫的可能性。
Malaria sporozoites invade the mosquito salivary glands and wait in the salivary duct until the next blood feeding. The mechanisms of the process and molecules involved in the salivary gland invasion remain largely unknown. To establish a robust salivary gland‐specific transgene expression in Anopheles stephensi, we obtained a salivary gland‐specific promoter for a gene encoding anopheline antiplatelet protein (AAPP). The aapp promoter is a female salivary gland‐specific and blood meal‐inducible strong promoter. Using this promoter, we generated a transgenic An. stephensi expressing abundant Discosoma sp. red fluorescent protein (DsRed) in the distal‐lateral lobes of the glands, where the sporozoites invade preferentially. These results open up the possibilities of elucidating salivary gland–parasite interactions and generating transgenic mosquitoes refractory to parasites.