Dissection of barley chromosome 5H in common wheat
Dissection of barley chromosome 5H in common wheat
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DOI：
10.1266/ggs.82.123
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发表时间：
2007-04-01
影响因子：
1.1
通讯作者：
Endo, Takashi R.
中科院分区：
文献类型：
--
作者：
Ashida, Taizo;Nasuda, Shuhei;Endo, Takashi R.
用遗传方法或杀配子体系统对添加到普通小麦上的大麦染色体5H进行解剖。首先，我们在携带配子体染色体的普通小麦5H附加系的后代中诱导染色体断裂，并从细胞学上筛选与5H有关的染色体结构变化，如缺失和易位。其次，我们筛选这些植物的后代，建立普通的小麦品系，这些品系携带结构改变的染色体，其中包含被解剖的5H的单个片段。利用23个具有代表性的5H解剖系，我们物理定位了97个大麦EST标记5H。est分布在23个解剖片段断点之间5H的20个区域，沿染色体分布较为均匀，在长臂远端区域频率明显较高。反过来，ESTs允许我们区分解剖的5H片段的断点。我们通过PCR（聚合酶链反应）和原位杂交证明，这些解剖的5H片段在解剖系中稳定地传播。我们讨论了5H解剖线对DNA标记物物理定位的有用性。这些5H解剖线可从日本国家生物资源项目-小麦获得。
We dissected barley chromosome 5H added to common wheat by a genetic method or the gametocidal system. Firstly, we induced chromosomal breaks in the offspring of a 5H addition line of common wheat carrying a gametocidal chromosome and cytologically screened for plants with structural chromosomal changes involving 5H, such as deletions and translocations. Secondly, we screened the progeny of such plants to establish common wheat lines carrying structurally changed chromosomes containing single segments of the dissected 5H. Using 23 representative 5H dissection lines, we physically mapped 97 barley EST markers assigned to 5H. The ESTs fell into 20 regions of 5H between the breakpoints of the 23 dissected segments, distributing rather evenly along the chromosome, with significantly higher frequency in the distal region of the long arm. The ESTs, in turn, allowed us to distinguish the breakpoints of dissected 5H segments. We demonstrated by PCR (polymerase chain reaction), as well as by in situ hybridization, that these dissected 5H segments were stably transmitted in the dissection lines. We discuss the usefulness of the 5H dissection lines for physical mapping of DNA markers. These 5H dissection lines are available from National BioResource Projects-Wheat, Japan.