Infrared Spectroscopy of Human Cells and Tissue. Part VII: FT-IR Microspectroscopy of DNase- and RNase-Treated Normal, Cirrhotic, and Neoplastic Liver Tissue
Infrared Spectroscopy of Human Cells and Tissue. Part VII: FT-IR Microspectroscopy of DNase- and RNase-Treated Normal, Cirrhotic, and Neoplastic Liver Tissue
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人体细胞和组织的红外光谱。
DOI：
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发表时间：
2000
期刊：
影响因子：
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通讯作者：
M. Diem
中科院分区：
文献类型：
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作者：
L. Chiriboga;H. Yee;M. Diem
在我们努力了解细胞和组织的红外光谱特征，以及正常和疾病状态之间发生的光谱变化时，我们以前报告了组织化学/免疫组织化学和光谱结果之间的详细关联。这些结果表明，与正常组织样本相比，肿瘤组织中的核酸光谱贡献增加。在本文中，这些研究扩展到分别报道了这些组织样本中DNA和RNA的光谱特征。这是通过选择性地消化组织切片中的DNA或RNA，分别留下含有核质/细胞质RNA的蛋白质基质或含有核DNA的蛋白质基质来实现的。这些结果表明，正常组织和肿瘤组织之间的光谱变化主要是由于肿瘤组织中DNA的增强签名所致。这种增强足够大，表明它最有可能是由于DNA可探测性的提高，而不是浓度的增加。
In our efforts to understand the infrared spectral features of cells and tissues, and the spectral changes occurring between normal and disease states, we reported previously a detailed correlation between histochemical/immunohistochemical and spectral results. These results suggested an increase of nucleic acid spectral contributions in neoplastic, as compared to normal, tissue samples. In the present paper, these studies are extended to report the spectral features of DNA and RNA separately in these tissue samples. This was accomplished by selectively digesting either DNA or RNA from tissue sections, leaving behind the protein matrix with nuclear/cytoplasmic RNA or the protein matrix with nuclear DNA, respectively. These results demonstrate that the spectral changes between normal and neoplastic tissue are mostly due to an enhanced signature of DNA in neoplastic tissue. This enhancement is sufficiently large to suggest that it is most likely due to an increased detectability of DNA, rather than an increase in concentration.