Acetylcholine and phorbol esters inhibit potassium currents evoked by adenosine and cAMP in Xenopus oocytes.
Acetylcholine and phorbol esters inhibit potassium currents evoked by adenosine and cAMP in Xenopus oocytes.
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乙酰胆碱和佛波酯抑制爪蟾卵母细胞中腺苷和 cAMP 引起的钾电流。
DOI：
10.1073/pnas.82.17.6001
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发表时间：
1985
影响因子：
11.1
通讯作者：
Lass,Y
中科院分区：
文献类型：
--
作者：
Dascal,N;Lotan,I;Gillo,B;Lester,HA;Lass,Y
在非洲爪蟾卵母细胞中，腺苷和其他嘌呤能激动剂诱导K+电导增加，细胞内应用cAMP完全模拟了这一过程。乙酰胆碱抑制腺苷、磷酸二酯酶抑制剂3-异丁基-1-甲基黄嘌呤或细胞内注射cAMP引起的K+电导增加。细胞内注射Ca2+或钙动员剂肌醇1,4,5-三磷酸不能模仿乙酰胆碱的这种作用。然而，腺苷和cAMP的反应被4 -phorbol 12,13-二丁酸酯和4 -phorbol 12-肉豆蔻酸13-乙酸酯抑制。这些结果表明，在非洲爪蟾卵母细胞中，毒蕈碱对嘌呤能和cAMP反应的抑制是通过激活依赖于磷脂的钙活化蛋白激酶（蛋白激酶C）来介导的。
In Xenopus laevis oocytes, adenosine and other purinergic agonists induce a K+-conductance increase that is fully mimicked by intracellular application of cAMP. Acetylcholine suppresses the K+-conductance increase caused by adenosine, by the phosphodiesterase inhibitor 3-isobutyl-1-methylxanthine, or by intracellular injection of cAMP. This effect of acetylcholine is not mimicked by intracellular injection of Ca2+ or of the Ca-mobilizing agent inositol 1,4,5-trisphosphate. However, adenosine and cAMP responses are inhibited by 4 beta-phorbol 12,13-dibutyrate and 4 beta-phorbol 12-myristate 13-acetate. These results suggest that, in Xenopus oocytes, the muscarinic inhibition of purinergic and cAMP responses is mediated through the activation of the phospholipid-dependent, Ca-activated protein kinase (protein kinase C).