Circulating Endothelial Progenitor Cells in Preterm Infants with Bronchopulmonary Dysplasia
Circulating Endothelial Progenitor Cells in Preterm Infants with Bronchopulmonary Dysplasia
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DOI：
10.1164/rccm.200812-1949oc
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发表时间：
2009-09-15
影响因子：
24.7
通讯作者：
Stronati, Mauro
中科院分区：
文献类型：
--
作者：
Borghesi, Alessandro;Massa, Margherita;Stronati, Mauro
基本原理新形式的支气管肺发育不良（BPD）的特征是肺部不成熟，肺泡和毛细血管发育后出生后出生，但肺血管形成受损的机制仍未完全理解。出生时循环的内皮祖细胞与鸟类的发展有关。胎龄小于32周或出生体重小于1,500 g的九十八名早产儿。通过在可用的脐带血的婴儿中，通过克隆分析评估了内皮菌落形成细胞（ECFC）。循环内皮细胞和造血细胞的比例是通过出生时流式细胞仪，48小时和生命的7天测量的。测量和主要结果：婴儿血液中的ECFC在后来发展BPD的婴儿中较低（中征[范围] [范围]： 0.00 [0.00-0.48] vs. 2.00 [0.00-21.87]； ECFC随着妊娠年龄的降低（r = 0.41; p = 0.02）的降低，但即使在极低的妊娠年龄，ECFC数量较高的婴儿受到保护，免受鸟类的保护。在患有和没有BPD的婴儿中，通过流式细胞仪研究的内皮和造血细胞亚群可比，出生后迅速降低。结论：ECFC在极低的妊娠年龄和妊娠期间的增加时较低。出生时表现出较低人数的非常早产的婴儿患BPD的风险增加。我们的发现表明，在极早出生后的ECFC降低可能与新BPD发生肺血管不成熟的风险有关。
Rationale The new form of bronchopulmonary dysplasia (BPD) is characterized by lung immaturity with disrupted alveolar and capillary development after extremely premature birth, but the mechanism of impaired lung vascular formation is still not completely understood.Objectives: We tested the hypothesis that reduced numbers of circulating endothelial progenitor cells at birth are associated with the development of BIRD.Methods: We studied ninety-eight preterm infants with gestational age of less than 32 weeks or a birth weight less than 1,500 g. Endothelial colony-forming cells (ECFCs) were assessed by clonogenic analysis in infants for whom cord blood was available. The proportion of circulating endothelial and hematopoietic cells was measured by flow cytometry at birth, at 48 hours, and at 7 days of life.Measurements and Main Results: ECFCs in cord blood were lower in infants who later developed BPD (median [range]: 0.00 [0.00-0.48] vs. 2.00 [0.00-21.87]; P = 0.002). ECFCs decreased with decreasing gestational age (r = 0.41; P = 0.02), but even at extremely low gestational ages, infants with higher numbers of ECFCs were protected from BIRD. The endothelial and hematopoietic cell subsets studied by flow cytometry were comparable in infants with and without BPD and rapidly decreased after birth.Conclusions: ECFCs are low at extremely low gestational ages and increase during gestation; extremely preterm infants who display lower numbers at birth have an increased risk of developing BPD. Our findings suggest that decreased ECFCs following extremely preterm birth may be associated with the risk for developing lung vascular immaturity characteristic of new BPD.