Oxidative damage and OGG1 expression induced by a combined effect of titanium dioxide nanoparticles and lead acetate in human hepatocytes
Oxidative damage and OGG1 expression induced by a combined effect of titanium dioxide nanoparticles and lead acetate in human hepatocytes
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DOI：
10.1002/tox.20682
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发表时间：
2012-10-01
影响因子：
4.5
通讯作者：
Zhou, Yikai
中科院分区：
文献类型：
--
作者：
Du, Hairong;Zhu, Xiaoling;Zhou, Yikai
二氧化钛（TiO 2）是一种广泛使用的纳米材料，可通过氧化应激引起生物损伤。在低浓度下，与单独的TiO 2或PbAc相比，TiO 2可以与乙酸铅（PbAc）相互作用以产生不同的毒性反应。在这项研究中，我们利用以下作为人类胚胎肝细胞（L02）的毒性反应的指标：活性氧（ROS），还原型谷胱甘肽（GSH），超氧化物歧化酶（SOD），和DNA加合物8-羟基脱氧鸟苷（8-OHdG）和8-氧代鸟嘌呤DNA糖基化酶同系物1（OGG 1）。这些被用来评估的氧化应激的TiO 2（在0.001，0.01，0.1，1，和10 μ g mL-1）与PbAc（1 μ g mL-1）混合的L02细胞没有光活化。与阴性对照相比（1份/千份二甲基亚砜），TiO 2与PbAc混合诱导ROS释放增加（0.001、0.01、0.1、1、10 μ g mL-1 TiO 2），细胞内SOD活性（0.1和0.01 μ g mL-1 TiO 2），GSH水平（0.011 μ g mL-1 TiO 2），8-OHdG水平（1和10 μ g mL-1 TiO 2），OGG 1表达（0.0011 μ g mL-1 TiO 2）和细胞毒性（0.1、1和10 μ g mL-1 TiO 2）。当单独用TiO 2或PbAc处理L02细胞时，ROS、GSH、SOD、8-OHdG或OGG 1水平没有显著变化。这些发现表明，在没有光活化的情况下，TiO 2和PbAc组合诱导L02细胞中的细胞毒性和氧化应激。(c)2011 Wiley Periodicals，Inc.环境毒理学，2012年。
Titanium dioxide (TiO2) is a widely used nanomaterial that can cause biological damage through oxidative stress. At low concentrations, TiO2 can interact with lead acetate (PbAc) to produce different toxic responses, compared with TiO2 or PbAc alone. In this study, we utilized the following as indicators of toxic responses in human embryo hepatocytes (L02): reactive oxygen species (ROS), reduced glutathione (GSH), superoxide dismutase (SOD), and the DNA adducts 8-hydroxydeoxyguanosine (8-OHdG) and 8-oxoguanine DNA glycosylase homolog 1 (OGG1). These were used to evaluate the oxidative stress of TiO2 (at 0.001, 0.01, 0.1, 1, and 10 mu g mL-1) mixed with PbAc (1 mu g mL-1) on L02 cells without photoactivation. Compared with the negative control (1 parts per thousand dimethyl sulfoxide), TiO2 mixed with PbAc induced increased release of ROS (at 0.001, 0.01, 0.1, 1, 10 mu g mL-1 TiO2), intracellular SOD activity (at 0.1 and 0.01 mu g mL-1 TiO2), GSH levels (at 0.011 mu g mL-1 TiO2), 8-OHdG levels (at 1 and 10 mu g mL-1 TiO2), OGG1 expression (at 0.0011 mu g mL-1 TiO2), and cytotoxicity (at 0.1, 1, and 10 mu g mL-1 TiO2) in L02 cells. There were no significant changes in ROS, GSH, SOD, 8-OHdG, or OGG1 levels when L02 cells were treated with TiO2 alone or PbAc alone. These findings indicate that TiO2 and PbAc in combination induce cytotoxicity and oxidative stress in L02 cells in the absence of photoactivation. (c) 2011 Wiley Periodicals, Inc. Environ Toxicol, 2012.