The effects of the interactions between amino acids on pyruvate kinase activity from the brain cortex of young rats
The effects of the interactions between amino acids on pyruvate kinase activity from the brain cortex of young rats
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DOI：
10.1016/j.ijdevneu.2005.06.002
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发表时间：
2005-10-01
影响因子：
1.8
通讯作者：
Wannmacher, CMD
中科院分区：
文献类型：
--
作者：
Feksa, LR;Cornelio, A;Wannmacher, CMD
考虑到丙酮酸激酶是脑中葡萄糖代谢和能量释放的关键酶，其活性可能受到某些氨基酸的调节，该酶活性的降低可能有助于代谢性疾病中积累的氨基酸（如苯丙氨酸、色氨酸和胱氨酸）引起的脑损伤。因此，本研究进行了调查，这些氨基酸对丙酮酸激酶活性的影响，在大脑皮层的大鼠。我们还研究了丝氨酸和丙氨酸对同一组织中丙酮酸激酶活性的影响。结果表明，苯丙氨酸，色氨酸，胱氨酸，丙氨酸和丝氨酸在酶的同一位点上起作用，苯丙氨酸，色氨酸和胱氨酸引起抑制，丙氨酸和丝氨酸阻止这种作用。胱氨酸还通过不同的机制抑制酶活性，可能作用于酶的巯基。考虑到这种酶是氨基酸代谢的一些代谢疾病中积累的氨基酸的靶点，它的抑制可能有助于在这些疾病中发现的脑损伤。(c)2005年ISDN。由爱思唯尔有限公司出版。保留所有权利。
Considering that pyruvate kinase activity, a crucial enzyme for glucose metabolism and energy liberation in brain, may be regulated by some amino acids, it is possible that diminution of this enzyme activity may contribute to the brain damage caused by amino acids accumulated in metabolic diseases, such as phenylalanine, tryptophan and cystine. Therefore, the present study was undertaken to investigate the effect of these amino acids on pyruvate kinase activity in the brain cortex of rats. We also investigated the effect of serine and alanine on pyruvate kinase activity in the same tissue. The results suggested that phenylalanine, tryptophan, cystine, alanine, and serine act at the same site on the enzyme, phenylalanine, tryptophan, and cystine causing inhibition, and alanine and serine preventing this effect. Cystine also inhibited the enzyme activity through a different mechanism, possibly acting on the enzyme thiol groups. Considering that this enzyme is a target for amino acids accumulated in some metabolic diseases of amino acid metabolism, it is possible that its inhibition may contribute to the brain damage found in these diseases. (c) 2005 ISDN. Published by Elsevier Ltd. All rights reserved.