Odorant-binding and chemosensory proteins identified in the antennal transcriptome of Adelphocoris suturalis Jakovlev
Odorant-binding and chemosensory proteins identified in the antennal transcriptome of Adelphocoris suturalis Jakovlev
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在 Adelphocoris suturalis Jakovlev 的触角转录组中鉴定出气味结合和化学感应蛋白
DOI：
10.1016/j.cbd.2016.03.001
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发表时间：
2017-12-01
影响因子：
3
通讯作者：
Zhang, Yong-Jun
中科院分区：
文献类型：
--
作者：
Cui, Huan-Huan;Gu, Shao-Hua;Zhang, Yong-Jun
中黑盲蝽（Adelphocoris suturalis Jakovlev）是一种对棉花等重要农作物造成严重危害的害虫。在昆虫的一生中，嗅觉是非常重要的。气味结合蛋白（odorant binding proteins，OBP）和化学感受蛋白（chemosensory proteins，CSP）是昆虫嗅觉信号传导过程中的两类可溶性蛋白质。在本研究中，共鉴定出16个OBP（12个经典OBP和4个plus-C OBP）和8个CSP。缝肌这些结合蛋白基因的性别和组织特异性的配置文件显示，16个OBP转录本中的13个在两种性别的触角中高度表达，4个OBP（AsutOBP 1，4，5和9）在雄性触角中的表达高于雌性触角。三种CSP（AsutCSP 1、4和5）在雌雄触角中特异表达，且AsutCSP 1在雄虫触角中的表达量高于雌虫触角。我们的研究结果确定了几个新的OBP和CSP基因，为进一步研究A。缝肌在分子水平上。(C)2016由Elsevier Inc.出版
Adelphocoris suturalis Jakovlev (Hemiptera: Miridae) is an insect pest that causes severe agricultural damage to cotton and many other important crops. In insects, olfaction is very important throughout their lifetime. There are two groups of small soluble proteins, named odorant binding proteins (OBPs) and chemosensory proteins (CSPs), which are suggested to participate in the initial biochemical recognition steps of insect olfactory signal transduction. In this study, a total of 16 OBPs (12 classical OBPs and 4 plus-C OBPs) and 8 CSPs, were identified in the antennal transcriptome of A. suturalis. The sex- and tissue-specific profiles of these binding protein genes showed that 13 of the 16 OBP transcripts were highly expressed in the antennae of both sexes, and 4 OBPs (AsutOBP1, 4, 5 and 9) were expressed higher in the male antennae compared to the female antennae. Three CSPs (AsutCSP1, 4 and 5) were expressed specifically in the antennae of both sexes, and AsutCSP1 was expressed higher in the male antennae than in the female antennae. Our findings identify several novel OBP and CSP genes for further investigation of the olfactory system of A. suturalis at the molecular level. (C) 2016 Published by Elsevier Inc.