Nucleoprotein-RNA Orientation in the Measles Virus Nucleocapsid by Three-Dimensional Electron Microscopy
Nucleoprotein-RNA Orientation in the Measles Virus Nucleocapsid by Three-Dimensional Electron Microscopy
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DOI：
10.1128/jvi.01459-10
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发表时间：
2011-02-01
影响因子：
5.4
通讯作者：
Gutsche, Irina
中科院分区：
文献类型：
--
作者：
Desfosses, Ambroise;Goret, Gael;Gutsche, Irina
用负染电镜分析重组麻疹病毒核蛋白-RNA（N-RNA）螺旋。胰蛋白酶消化和完整的核衣壳的三维重建耦合到对接的原子结构的呼吸道合胞病毒（RSV）的N-RNA亚基到电子显微镜密度图支持一个模型，将RNA的外部的螺旋和无序的C-末端结构域朝向螺旋内部，他们建议的位置的六个核苷酸相对于麻疹N原体。
Recombinant measles virus nucleoprotein-RNA (N-RNA) helices were analyzed by negative-stain electron microscopy. Three-dimensional reconstructions of trypsin-digested and intact nucleocapsids coupled to the docking of the atomic structure of the respiratory syncytial virus (RSV) N-RNA subunit into the electron microscopy density map support a model that places the RNA at the exterior of the helix and the disordered C-terminal domain toward the helix interior, and they suggest the position of the six nucleotides with respect to the measles N protomer.