GINS Is a DNA Polymerase ϵ Accessory Factor during Chromosomal DNA Replication in Budding Yeast*
GINS Is a DNA Polymerase ϵ Accessory Factor during Chromosomal DNA Replication in Budding Yeast*
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GINS 是芽殖酵母染色体 DNA 复制过程中的 DNA 聚合酶 ϵ 辅助因子*
DOI：
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发表时间：
2006
影响因子：
4.8
通讯作者：
A. Sugino
中科院分区：
文献类型：
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作者：
T. Seki;M. Akita;Y. Kamimura;Sachiko Muramatsu;H. Araki;A. Sugino
GINS是真核细胞中发现的蛋白质复合物，由Sld5p、Psf1p、Psf2p和Psf3p组成。GINS多肽在真核生物中高度保守，并且GINS复合物是酵母和爪蟾卵中染色体DNA复制所需的。本文报道了酿酒酵母GINS的分离纯化及其生化特性。结果表明，GINS与DNA聚合酶β（Poly β）全酶形成1：1的复合物，并极大地促进其体外催化活性。在GINS存在下，Pol β更具进行性，更容易从复制的DNA中解离，而在相同条件下，增殖细胞核抗原在体外轻微刺激Pol β。这些结果强烈地表明GINS是芽殖酵母染色体DNA复制过程中的一种Poll辅助蛋白。基于这些结果，我们提出了真核生物染色体复制叉的分子动力学模型。
GINS is a protein complex found in eukaryotic cells that is composed of Sld5p, Psf1p, Psf2p, and Psf3p. GINS polypeptides are highly conserved in eukaryotes, and the GINS complex is required for chromosomal DNA replication in yeasts and Xenopus egg. This study reports purification and biochemical characterization of GINS from Saccharomyces cerevisiae. The results presented here demonstrate that GINS forms a 1:1 complex with DNA polymerase ϵ (Pol ϵ) holoenzyme and greatly stimulates its catalytic activity in vitro. In the presence of GINS, Pol ϵ is more processive and dissociates more readily from replicated DNA, while under identical conditions, proliferating cell nuclear antigen slightly stimulates Pol ϵ in vitro. These results strongly suggest that GINS is a Pol ϵ accessory protein during chromosomal DNA replication in budding yeast. Based on these results, we propose a model for molecular dynamics at eukaryotic chromosomal replication fork.
影响因子：
4.8
作者：
Podust, VN;Chang, LS;Fanning, E
通讯作者：
Fanning, E
DOI：
10.1073/pnas.94.21.11244
发表时间：
1997-10-14
影响因子：
11.1
作者：
Zuo, SJ;Gibbs, E;Hurwitz, J
通讯作者：
Hurwitz, J