Comparison of Two Multiplex Methods for Detection of Respiratory Viruses: FilmArray RP and xTAG RVP
Comparison of Two Multiplex Methods for Detection of Respiratory Viruses: FilmArray RP and xTAG RVP
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DOI：
10.1128/jcm.02582-10
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发表时间：
2011-07-01
影响因子：
9.4
通讯作者：
Houck, Herbert J.
中科院分区：
文献类型：
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作者：
Rand, Kenneth H.;Rampersaud, Howard;Houck, Herbert J.
我们比较了FilmArray RP（爱达荷州Technology，Inc.，湖城，UT）和xTAG RVP（Luminex Corporation，多伦多，加拿大）多重呼吸道病毒PCR方法，用于在进行标准病毒培养和抗原检测方法后，检测在-70 ℃下冷冻的一组200份患者标本中的呼吸道病毒。这两种系统检测到的病毒比传统方法多40%至50%，主要是鼻病毒和人类偏肺病毒。与xTAG RVP相比，FilmArray RP检测到的单独病毒或混合感染的病毒总数显著更多，呼吸道合胞病毒也多出21.6%。xTAG RVP需要5至6小时，其中动手操作时间为2.5至3小时，而FilmArray RP需要约1小时，其中动手操作时间为3至5分钟，因此操作起来更加容易。
We compared the FilmArray RP (Idaho Technology, Inc., Salt Lake City, UT) and the xTAG RVP (Luminex Corporation, Toronto, Canada) multiplex respiratory virus PCR methods for the detection of respiratory viruses in a set of 200 patient specimens frozen at -70 degrees C after standard viral culture and antigen detection methods were done. Both systems detected between 40 to 50% more viruses than traditional methods, primarily rhinoviruses and human metapneumovirus. The FilmArray RP detected significantly more total viruses either alone or as part of mixed infections than the xTAG RVP, as well as an additional 21.6% more respiratory syncytial viruses. The xTAG RVP requires 5 to 6 h with 2.5 to 3 h of hands-on time, while the FilmArray RP takes about an hour with 3 to 5 min of hands-on time, making it much easier to perform.