Exploring the Mechanism of Ling-Gui-Zhu-Gan Decoction in Ventricular Remodeling after Acute Myocardial Infarction Based on UPLC and In Vivo Experiments.
Exploring the Mechanism of Ling-Gui-Zhu-Gan Decoction in Ventricular Remodeling after Acute Myocardial Infarction Based on UPLC and In Vivo Experiments.
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DOI：
10.1155/2022/8593176
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发表时间：
2022
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影响因子：
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Ventricular remodeling (VR) after acute myocardial infarction (AMI) is an important pathophysiological basis for the development of chronic heart failure (CHF). At present, Ling-Gui-Zhu-Gan decoction (LGZGD) has been widely reported in the clinical treatment and basic research of cardiovascular diseases (CVDs), such as myocardial infarction, heart failure, and angina pectoris. However, the mechanism of LGZGD against VR after AMI remains unclear. Ultra-performance liquid chromatography (UPLC) was applied to investigate the major constituents of LGZGD, and molecular docking was used to predict the targets on the NLRP3/Caspase-1/GSDMD signaling pathway. In vivo, histological changes in the myocardium were visualized using HE staining and Masson staining, and cardiomyocyte apoptosis was detected using TUNEL. IL-1β activity in rat serum was determined by ELISA. Finally, NLRP3, Caspase-1, and GSDMD expressions were analyzed through RT-qPCR and Western blotting. The results showed that 8 authentic reference substances have been detected in LGZGD. Molecular docking showed that the major chemical constituents of LGZGD had a good binding activity with NLRP3, Caspase-1, and GSDMD. Our results showed that LGZGD treatment markedly improved cardiac pathology, decreased cardiomyocyte apoptosis, reduced IL-1β activity, and regulated the expression of genes and proteins related to the NLRP3/Caspase-1/GSDMD signal pathway. These results suggest that LGZGD protects against VR after AMI through NLRP3/Caspase-1/GSDMD signal pathway.
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DOI：
10.1007/978-1-4939-7000-1_26
发表时间：
2017
期刊：
Methods in molecular biology (Clifton, N.J.)
影响因子：
--
作者：
Burley SK;Berman HM;Kleywegt GJ;Markley JL;Nakamura H;Velankar S
通讯作者：
Velankar S
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影响因子：
6.1
作者：
Li, Ziyin;Chi, Huiqin;Yang, Xingfen
通讯作者：
Yang, Xingfen
选择性 NLRP3 炎症小体抑制剂 MCC950 可减少心肌梗塞小鼠模型中的心肌纤维化并改善心脏重塑。
影响因子：
5.6
作者：
Gao, Rifeng;Shi, Huairui;Tang, Yanhua
通讯作者：
Tang, Yanhua
DOI：
10.1152/ajpheart.00603.2017
发表时间：
2018-09-01
影响因子：
4.8
作者：
Bradley, Jessica M.;Spaletra, Pablo;Lefer, David T.
通讯作者：
Lefer, David T.
影响因子：
9.3
作者：
Li Y;Song W;Tong Y;Zhang X;Zhao J;Gao X;Yong J;Wang H
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Wang H