Optimisation of a lozenge-based sensor for detecting impending blockage of urinary catheters.
Optimisation of a lozenge-based sensor for detecting impending blockage of urinary catheters.
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DOI：
10.1016/j.bios.2021.113775
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发表时间：
2021-11
影响因子：
12.6
通讯作者：
R. Heylen;Max Branson;Lauren Gwynne;B. Patenall;Nina Hauschildt;J. Urie;J. Mercer-Chalmers;
中科院分区：
文献类型：
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作者：
R. Heylen;Max Branson;Lauren Gwynne;B. Patenall;Nina Hauschildt;J. Urie;J. Mercer-Chalmers;
由于微生物的脲酶活性，由脲酶阳性微生物引起的导尿管相关尿路感染更有可能引起导尿管堵塞。导管堵塞可能是危险的，并增加严重感染的风险，如败血症。脲是变形杆菌中的一种毒力因子，可引起尿液pH值升高，从而导致堵塞。这里介绍了一种优化的生物传感器“含片”，它能够检测即将发生的导管阻塞。这种含片经过优化，易于生产和商业化。它在一个有生理代表性的尿路导尿模型中起传感器的作用，在导管堵塞之前提供6.7小时的警告。该含片在健康人尿液中稳定，可经环氧乙烷消毒，供临床使用。临床上，该含片将提供导管即将阻塞的明显指示，使临床干预更快，从而降低与阻塞相关的发病率和死亡率。
Catheter-associated urinary tract infections resulting from urease-positive microorganisms are more likely to cause a urinary catheter blockage owing to the urease activity of the microbes. Catheter blockage can be dangerous and increases the risk of severe infections, such as sepsis. Ureases, a virulence factor inProteus mirabilis, cause an increase in urine pH - leading to blockage. An optimised biosensor “lozenge” is presented here, which is able to detect impending catheter blockage. This lozenge has been optimised to allow easy manufacture and commercialisation. It functions as a sensor in a physiologically representative model of a catheterised urinary tract, providing 6.7 h warning prior to catheter blockage. The lozenge is stable in healthy human urine and can be sterilized for clinical use by ethylene oxide. Clinically, the lozenge will provide a visible indication of impending catheter blockage, enabling quicker clinical intervention and thus reducing the morbidity and mortality associated with blockage.