SATB1 makes a complex with p300 and represses gp91phox promoter activity
SATB1 makes a complex with p300 and represses gp91phox promoter activity
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DOI：
10.1111/j.1348-0421.2003.tb03438.x
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发表时间：
2003-01-01
影响因子：
2.6
通讯作者：
Nakamura, M
中科院分区：
文献类型：
--
作者：
Fujii, Y;Kumatori, A;Nakamura, M
gp 91（phox）是吞噬细胞NADPH氧化酶的关键组分，其表达受与其近端启动子结合的多种因子调节。两种核基质附着区（MAR）结合蛋白，特异性富AT结合蛋白1（SATB 1）和CCAAT置换蛋白（CDP），已被报道为gp 91（phox）基因阻遏物的罕见例子。然而，它们各自的作用以及与启动子中其他因子的相互作用尚未详细阐明。我们对这两个抑制蛋白进行了研究，发现它们分别识别该基因的bp-115到bp-106片段，并获得了以下结果：1. SATB 1主要与p300形成复合物，而不考虑DNA的存在。2. SATB 1/p300复合物与5'上游AT富集区（bp-115至bp-106）结合抑制gp 91（phox）启动子活性，CDP与AT富集区下游的CCAAT元件结合可部分释放被抑制的活性。我们的研究结果意味着p300在SATB 1相关的全球转录调控中的新作用。
The expression of gp91(phox), the key component of the phagocyte NADPH oxidase, is regulated by various factors binding to its proximal promoter. Two nuclear matrix attachment region (MAR)-binding proteins, special AT-rich binding protein 1 (SATB1) and CCAAT displacement protein (CDP), have been reported as rare examples of gp91(phox) gene repressors. However, their individual roles and interactions with other factors in the promoter have not been elucidated in detail. We have focused on these two repressive proteins recognizing the bp -115 to bp -106 segment of the gene and obtained the following results: 1. SATB1 makes a complex, mainly with p300, regardless of the presence of DNA. 2. SATB1/p300 complex binding to the 5' upstream AT-rich region in the bp -115 to bp -106 segment represses the gp91(phox) promoter activity, and the repressed activity is partially released by CDP binding to the CCAAT element directly downstream of the AT-rich region. Our findings imply a novel role for p300 in SATB1-associated global transcription regulation.