BmDJ-1 is a key regulator of oxidative modification in the development of the silkworm, Bombyx mori.
BmDJ-1 is a key regulator of oxidative modification in the development of the silkworm, Bombyx mori.
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DOI：
10.1371/journal.pone.0017683
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发表时间：
2011-03-24
期刊：
影响因子：
3.7
通讯作者：
Satoh J
中科院分区：
文献类型：
--
作者：
Tabunoki H;Ode H;Banno Y;Katsuma S;Shimada T;Mita K;Yamamoto K;Sato R;Ishii-Nozawa R;Satoh J
我们从家蚕幼虫脑中克隆了家蚕DJ-1蛋白（BmDJ-1）的cDNA。BmDJ-1由190个氨基酸组成，由672个核苷酸编码。北方印迹分析表明BmDJ-1转录为一个756 bp的mRNA，有一个异构体。逆转录酶（RT）-PCR实验表明，BmDJ-1存在于第4天五龄幼虫的脑、脂肪体、马尔皮基管、卵巢和睾丸中，但在丝腺和血细胞中仅少量存在。免疫组化结果表明，BmDJ-1在仔鱼至成虫的脑、中肠、脂肪体、马氏管、睾丸和卵巢中均有表达。我们发现BmDJ-1在五龄幼虫到成虫发育阶段具有独特的表达模式。我们使用鱼藤酮（ROT）在第3天的5龄幼虫中评估了BmDJ-1的抗氧化功能。给第3天的5龄幼虫施用ROT，连同外源性（BmNPV-BmDJ-1提前4天感染）BmDJ-1，在BmDJ-1组中产生比对照组显著更低的24小时死亡率。2D-PAGE显示，BmDJ-1在BmN 4细胞中的等电点（pI）在ROT刺激后向酸性形式移动。在检查其对血淋巴中BmDJ-1的表达水平的影响的因素中，一氧化氮（NO）浓度基于显著的发育阶段依赖性变化来确定。硝酸异山梨酯（ISDN），这是一个NO供体，BmN 4细胞的管理产生的BmDJ-1的表达增加相比，控制。这些结果表明，BmDJ-1可能控制细胞中由于NO的氧化应激，并作为B的发育调节因子。桑。
We cloned cDNA for the Bombyx mori DJ-1 protein (BmDJ-1) from the brains of larvae. BmDJ-1 is composed of 190 amino acids and encoded by 672 nucleotides. Northern blot analysis showed that BmDJ-1 is transcribed as a 756-bp mRNA and has one isoform. Reverse transcriptase (RT)-PCR experiments revealed that the BmDJ-1 was present in the brain, fatbody, Malpighian tubule, ovary and testis but present in only low amounts in the silkgland and hemocyte of day 4 fifth instar larvae. Immunological analysis demonstrated the presence of BmDJ-1 in the brain, midgut, fatbody, Malpighian tubule, testis and ovary from the larvae to the adult. We found that BmDJ-1 has a unique expression pattern through the fifth instar larval to adult developmental stage. We assessed the anti-oxidative function of BmDJ-1 using rotenone (ROT) in day 3 fifth instar larvae. Administration of ROT to day 3 fifth instar larvae, together with exogenous (BmNPV-BmDJ-1 infection for 4 days in advance) BmDJ-1, produced significantly lower 24-h mortality in BmDJ-1 groups than in the control. 2D-PAGE revealed an isoelectric point (pI) shift to an acidic form for BmDJ-1 in BmN4 cells upon ROT stimulus. Among the factors examined for their effects on expression level of BmDJ-1 in the hemolymph, nitric oxide (NO) concentration was identified based on dramatic developmental stage-dependent changes. Administration of isosorbide dinitrate (ISDN), which is an NO donor, to BmN4 cells produced increased expression of BmDJ-1 compared to the control. These results suggest that BmDJ-1 might control oxidative stress in the cell due to NO and serves as a development modulation factor in B. mori.
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影响因子：
2.9
作者：
Anderson, Peter C.;Daggett, Valerie
通讯作者：
Daggett, Valerie
DOI：
10.1073/pnas.0511270103
发表时间：
2006-03-21
影响因子：
11.1
作者：
Ohnishi, A;Hull, JJ;Matsumoto, S
通讯作者：
Matsumoto, S
影响因子：
4.8
作者：
Tabunoki, H;Sugiyama, H;Tsuchida, K
通讯作者：
Tsuchida, K
影响因子：
4.8
作者：
Tao, X;Tong, L
通讯作者：
Tong, L
影响因子：
46.9
作者：
Tomita, M;Munetsuna, H;Yoshizato, K
通讯作者：
Yoshizato, K