Identification of suppressors of cytokine signaling (SOCS) proteins in human gestational tissues: Differential regulation is associated with the onset of labor
Identification of suppressors of cytokine signaling (SOCS) proteins in human gestational tissues: Differential regulation is associated with the onset of labor
复制标题
DOI：
10.1210/jc.87.3.1094
复制
发表时间：
2002-03-01
影响因子：
5.8
通讯作者：
Mitchell, MD
中科院分区：
文献类型：
--
作者：
Blumenstein, M;Bowen-Shauver, JM;Mitchell, MD
由胎盘和胎膜分泌的炎性细胞因子被认为在分娩开始中起重要作用。细胞因子信号传导抑制因子（SOCS）蛋白通过几种细胞因子调节信号转导，这些细胞因子已被报道影响妊娠组织。然而，SOCS蛋白在人类妊娠组织中的存在、分布和作用尚未被描述。采用逆转录酶(RT)-PCR和Western blot技术分别检测了人绒毛胎盘、羊膜和蜕毛中SOCS1、SOCS2和SOCS3 mRNA和蛋白的表达情况。在自然分娩、阴道分娩或剖宫产（分娩前）后的足月无并发症妊娠中获得组织。与劳动状态无关，SOCS1、SOCS2和SOCS3的信使rna在所有组织类型中均有表达。然而，SOCS蛋白仅在绒毛胎盘中检测到，在一例蜕毛膜中，分娩与绒毛胎盘和蜕毛膜样品中SOCS1和SOCS3蛋白的表达缺失相关，分娩后SOCS2蛋白带的大小略有增加，这可能表明SOCS2翻译后修饰。妊娠组织中SOCS蛋白表达的减少可能提供了一种机制，通过这种机制，炎症细胞因子进入炎症变化的正反馈循环，从而导致分娩。
Inflammatory cytokines secreted by the placenta and fetal membranes are believed to play an important role in the initiation of parturition. The suppressor of cytokine signaling (SOCS) proteins regulate signal transduction by several cytokines that have been reported to affect gestational tissues. The presence, distribution and roles of SOCS proteins, however, have not been described in human gestational tissues. Using reverse transcriptase (RT)-PCR and Western blot analysis we investigated the expression of SOCS1, SOCS2 and SOCS3 mRNA and protein, respectively, by human villous placenta, amnion and choriodecidua (n=3-4). Tissues were obtained from uncomplicated pregnancies at term after either spontaneous labor and vaginal delivery or caesarian section (before labor). Messenger RNAs for SOCS1, SOCS2 and SOCS3 were expressed in all tissue types, irrespective of labor status. SOCS proteins were, however, only detectable in villous placenta and in one case in the choriodecidua, Labor was associated with abrogated expression of SOCS1 and SOCS3 proteins in villous placenta and the choriodecidua sample, Following labor the band for SOCS2 protein increased slightly in size which may indicate post-translational modification of SOCS2. Reduced expression of SOCS proteins in gestational tissues may provide a mechanism by which inflammatory cytokines enter into a positive feedback loop of inflammatory changes leading to delivery.