Multifunctional polyelectrolyte multilayer films:: Combining mechanical resistance, biodegradability, and bioactivity
Multifunctional polyelectrolyte multilayer films:: Combining mechanical resistance, biodegradability, and bioactivity
复制标题
DOI：
10.1021/bm060765k
复制
发表时间：
2007-01-01
影响因子：
6.2
通讯作者：
Picart, Catherine
中科院分区：
文献类型：
--
作者：
Schneider, Aurore;Vodouhe, Constant;Picart, Catherine
交联聚电解质多层膜（CL PEM）具有更高的刚性，并且比天然（例如，非交联）膜具有更强的机械抗性。然而，它们仍然是可生物降解的，这使它们成为生物医学应用的有趣候选者。在这项研究中，研究了CL PEM薄膜的多功能特性，如(i)耐机械，（ii）可生物降解和（iii）生物活性薄膜。为此，我们通过药物的简单扩散研究了CL壳聚糖/透明质酸（CHI/HA）和聚l -赖氨酸/透明质酸（PLL/HA）薄膜上的药物负载。选择双氯芬酸钠和紫杉醇作为模型药物，成功地将其装入薄膜中。研究了（CHI/HA）膜层数和交联剂浓度对双氯芬酸负载的影响。双氯芬酸在10 h左右从膜中释放，紫杉醇也在CL膜中扩散。在CL膜中加载后，其活性保持不变，细胞活力在3天内可降低约55%。这种简单的方法可以应用于其他类型的交联膜和其他药物。这些结果证明，设计多功能多层膜是可能的，它将机械阻力、生物降解性和生物活性特性结合在一个单一的PEM结构中。
Cross-linked polyelectrolyte multilayer films (CL PEM) have an increased rigidity and are mechanically more resistant than native ( e. g., uncrosslinked) films. However, they are still biodegradable, which make them interesting candidates for biomedical applications. In this study, CL PEM films have been explored for their multifunctional properties as (i) mechanically resistant, (ii) biodegradable, and (iii) bioactive films. Toward this end, we investigated drug loading into CL chitosan/hyaluronan (CHI/HA) and poly(L-lysine)/ hyaluronan (PLL/HA) films by simple diffusion of the drugs. Sodium diclofenac and paclitaxel were chosen as model drugs and were successfully loaded into the films. The effect of varying the number of layers in the (CHI/HA) films as well as the cross-linker concentration on diclofenac loading were studied. Diclofenac was released from the film in about 10 h. Paclitaxel was also found to diffuse within CL films. Its activity was maintained after loading in the CL films, and cellular viability could be reduced by about 55% over 3 days. Such a simple approach may be applied to other types of cross-linked films and to other drugs. These results prove that it is possible to design multifunctional multilayer films that combine mechanical resistance, biodegradability, and bioactivity properties into a single PEM architecture.