HEIP1 regulates crossover formation during meiosis in rice
HEIP1 regulates crossover formation during meiosis in rice
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HEIP1 调节水稻减数分裂过程中的交叉形成
DOI：
10.1073/pnas.1807871115
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发表时间：
2018-10-16
影响因子：
11.1
通讯作者：
Cheng, Zhukuan
中科院分区：
文献类型：
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作者：
Li, Yafei;Qin, Baoxiang;Cheng, Zhukuan
显着性交叉(CoS)确保了减数分裂过程中同源染色体的准确分离。如果不能创造适当数量的交叉，可能会导致遗传物质在子代细胞中的分配不均，从而导致不育。CO蛋白是在减数分裂过程中特异定位于交叉部位的蛋白，在CO的形成过程中起着关键作用。在这里，我们在水稻中发现了一个CO蛋白，命名为HEI10相互作用蛋白1(HEIP1)，它在高等植物中是保守的。我们的结果揭示了它在减数分裂过程中控制CO的可能分子机制。在减数分裂过程中，双链断裂(DSB)的数量远远超过最终的交叉(CoS)数量。因此，确定哪些DSB修复为CoS所涉及的蛋白质对于理解CO的调控机制是至关重要的。在整个物种中，HEI10相关蛋白在CO的形成中发挥着重要作用。在这里，通过酵母双杂交系统筛选与HEI10相互作用的蛋白，我们在水稻中鉴定出一个CO蛋白HEI10相互作用蛋白1(HEIP1)。HEIP1以动态方式沿着减数分裂染色体与HEI10共定位，并特别定位于从粗线期晚期到双线期的交叉部位。在这两种蛋白质中，HEIP1的负载需要HEI10，反之亦然。此外，HEIP1基因的突变导致交叉频率的严重降低，而早期的同源重组过程不受干扰，突触正常进行。HEIP1与ZIP4和MSH5直接相互作用。此外，HEIP1的加载依赖于ZIP4，而不是MER3、MSH4或MSH5。综上所述，我们的结果表明，HEIP1可能是ZMM基团的一个成员，并且是调节CO形成的关键元件。
Significance Crossovers (COs) ensure the accurate segregation of homologous chromosomes during meiosis. Failure to create the right number of crossovers may lead to unequal distribution of genetic materials to daughter cells and sterility. CO proteins, which specially localize to crossover sites during meiosis, play critical roles in CO formation. Here, we identify a CO protein in rice, named HEI10 Interaction Protein 1 (HEIP1), which is conserved in higher plants. Our results reveal its possible molecular mechanism for CO control in meiosis. During meiosis, the number of double-strand breaks (DSBs) far exceeds the final number of crossovers (COs). Therefore, to identify proteins involved in determining which of these DSBs repaired into COs is critical in understanding the mechanism of CO control. Across species, HEI10-related proteins play important roles in CO formation. Here, through screening for HEI10-interacting proteins via a yeast two-hybrid system, we identify a CO protein HEI10 Interaction Protein 1 (HEIP1) in rice. HEIP1 colocalizes with HEI10 in a dynamic fashion along the meiotic chromosomes and specially localizes onto crossover sites from late pachytene to diplotene. Between these two proteins, HEI10 is required for the loading of HEIP1, but not vice versa. Moreover, mutations of the HEIP1 gene cause the severe reduction of chiasma frequency, whereas early homologous recombination processes are not disturbed and synapsis proceeds normally. HEIP1 interacts directly with ZIP4 and MSH5. In addition, the loading of HEIP1 depends on ZIP4, but not on MER3, MSH4, or MSH5. Together, our results suggest that HEIP1 may be a member of the ZMM group and acts as a key element regulating CO formation.