A novel preadipocyte cell line established from mouse adult mature adipocytes
A novel preadipocyte cell line established from mouse adult mature adipocytes
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DOI：
10.1016/j.bbrc.2004.07.055
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发表时间：
2004-09-03
影响因子：
3.1
通讯作者：
Kano, K
中科院分区：
文献类型：
--
作者：
Yagi, K;Kondo, D;Kano, K
我们从小鼠成年成熟脂肪细胞中建立了一种新的前脂肪细胞系。从脂肪组织中分离成熟脂肪细胞，只取成熟脂肪细胞的流动群体。分离的成熟脂肪细胞去分化为成纤维细胞样细胞。体外实验表明，细胞经过20多次传代后可再分化为成熟脂肪细胞。体内移植研究还表明，细胞具有完全分化为成熟脂肪细胞的潜力，而来源于小鼠胚胎的3T3-L1前脂肪细胞系尚未显示出这一点。我们进一步分析了关键脂肪调控基因的表达谱，如过氧化物酶体增殖激活受体或CCAA Tl增强结合蛋白基因家族。我们得出的结论是，我们的细胞系可以作为3T3-L1的首选替代品，可能更多地反映成熟脂肪细胞的特征，因为该细胞系实际上来源于成人成熟脂肪细胞。(C) 2004爱思唯尔公司版权所有。
We have established a novel preadipocyte cell line from mouse adult mature adipocytes. The mature adipocytes were isolated from fat tissues by taking only the floating population of mature fat cells. The isolated mature adipocytes were de-differentiated into fibroblast-like cells. The in vitro studies showed that the cells could re-differentiate into mature adipocytes after over 20 passages. The in vivo transplantation study also demonstrated that the cells had the full potential to differentiate into mature adipocytes, which has not been shown for the 3T3-L1 preadipocyte cell line derived from mouse embryo. We have further analyzed the expression profile of key fat regulatory genes such as the peroxisome proliferator-activated receptory or CCAA Tl enhancer-binding protein gene families. We conclude that our cell line could be used as a preferred alternative to 3T3-L1, potentially reflecting the characteristics of mature adipocytes more, since the cell line is actually derived from adult mature adipocytes. (C) 2004 Elsevier Inc. All rights reserved.