Identification and Analysis of microRNAs in the SAM and Leaves of Populus tomentosa
Identification and Analysis of microRNAs in the SAM and Leaves of Populus tomentosa
复制标题
毛白杨 SAM 和叶片中 microRNA 的鉴定与分析
DOI：
10.3390/f10020130
复制
发表时间：
2019-02-01
期刊：
影响因子：
2.9
通讯作者：
Jin, Biao
中科院分区：
文献类型：
--
作者：
Cui, Jiawen;Lu, Weichao;Jin, Biao
茎顶端分生组织（SAM）是位于植物顶端的一个重要组织，它能持续生长和分化发育成地上部分。SAM的发生受到一系列复杂的分子调控网络的控制，其中microRNA（miRNAs）及其靶基因起着关键作用。然而，对木本植物中的这些miRNAs知之甚少。本研究利用小分子RNA（small RNA，sRNA）测序技术构建了4个毛白杨茎尖和成熟叶组织的miRNA文库，鉴定了99个已知的miRNA家族。此外，193个已知的miRNAs，包括植物激素，发育和细胞过程相关的miRNAs，表现出显着的差异表达。有趣的是，miR172，miR164和miR393表达的实时定量逆转录聚合酶链反应（PCR）分析显示，在茎尖发育过程中表达模式发生了显着变化。这些miRNAs的靶基因参与调节激素反应和干细胞功能。特别是，参与茎尖干细胞维持的miR172靶标APETALA2（AP2）在发育的初始活跃阶段特异性表达。这些发现为研究miRNAs在树木SAM发育和分化中的调控机制提供了新的思路。
The shoot apical meristem (SAM) is a crucial tissue located at the tops of plants which can continually grow and differentiate to develop into all aboveground parts. SAM development is controlled by a series of complicated molecular regulation networks, among which microRNAs (miRNAs) and their target genes play key roles. However, little is known about these miRNAs in woody plants. In this study, we used small RNA (sRNA) sequencing to build four libraries derived from shoot tips and mature leaf tissues of Populus tomentosa, and identified 99 known miRNA families. In addition, 193 known miRNAs, including phytohormone-, developmental-, and cellular process-related miRNAs, showed significant differential expression. Interestingly, quantitative real-time reverse transcription polymerase chain reaction (PCR) analysis of miR172, miR164, and miR393 expression showed marked changes in expression patterns during the development of shoot tips. The target genes of these miRNAs were involved in the regulation of hormone responses and stem cell function. In particular, the miR172 target APETALA2 (AP2), involved in the maintenance of stem cells in the shoot apex, was expressed specifically during the initial active stage of development. These findings provide new insights into the regulatory mechanisms of miRNAs involved in SAM development and differentiation in tree species.