RCCS bioreactor-based modelled microgravity induces significant changes on in vitro 3D neuroglial cell cultures.
RCCS bioreactor-based modelled microgravity induces significant changes on in vitro 3D neuroglial cell cultures.
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DOI：
10.1155/2015/754283
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发表时间：
2015
影响因子：
--
通讯作者：
Mariggiò MA
中科院分区：
文献类型：
--
作者：
Morabito C;Steimberg N;Mazzoleni G;Guarnieri S;Fanò-Illic G;Mariggiò MA
为了研究微重力对细胞-细胞相互作用的影响，我们提出了一个人源性神经/神经胶质细胞的三维体外模型。利用旋转细胞培养系统(RCCS)生物反应器模拟微重力环境，分析了GL15胶质样细胞和神经元样SH-SY5Y细胞在三维单独培养(单型集合体)和共培养(异型集合体)中的形态功能特征。在培养的2周内，所有细胞聚集体内的细胞存活均得以维持。此外，与传统的静态单层细胞相比，在模拟微重力条件下培养的细胞聚集体显示出特定分化标志物(例如GL15细胞：GFAP、S100B；SH-SY5Y细胞：GAP43)的表达增加，并调节了细胞与细胞之间的功能相互作用(例如，N-CAM和Cx43的表达和定位)。总之，该培养模型在分子、生化和形态水平上开启了广泛的具体研究，为体外研究神经系统细胞组件对微重力环境条件的动态相互作用和响应提供了重要工具。
We propose a human-derived neuro-/glial cell three-dimensional in vitro model to investigate the effects of microgravity on cell-cell interactions. A rotary cell-culture system (RCCS) bioreactor was used to generate a modelled microgravity environment, and morphofunctional features of glial-like GL15 and neuronal-like SH-SY5Y cells in three-dimensional individual cultures (monotypic aggregates) and cocultures (heterotypic aggregates) were analysed. Cell survival was maintained within all cell aggregates over 2 weeks of culture. Moreover, compared to cells as traditional static monolayers, cell aggregates cultured under modelled microgravity showed increased expression of specific differentiation markers (e.g., GL15 cells: GFAP, S100B; SH-SY5Y cells: GAP43) and modulation of functional cell-cell interactions (e.g., N-CAM and Cx43 expression and localisation). In conclusion, this culture model opens a wide range of specific investigations at the molecular, biochemical, and morphological levels, and it represents an important tool for in vitro studies into dynamic interactions and responses of nervous system cell components to microgravity environmental conditions.
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