Nanostructured zinc oxide platform for mycotoxin detection
Nanostructured zinc oxide platform for mycotoxin detection
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DOI：
10.1016/j.bioelechem.2009.06.014
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发表时间：
2010-02-01
影响因子：
5
通讯作者：
Malhotra, B. D.
中科院分区：
文献类型：
--
作者：
Ansari, Anees A.;Kaushik, Ajeet;Malhotra, B. D.
将纳米氧化锌（Nano-ZnO）薄膜沉积在氧化铟锡（ITO）玻璃板上，用于兔免疫球蛋白抗体（r-IgG）和牛血清白蛋白（BSA）的共固定，并用于赭曲霉毒素A（OTA）的检测。X射线衍射（XRD）结果表明，纳米ZnO的平均粒径约为5.0 nm。采用傅里叶变换红外光谱（FTIR）、扫描电镜（SEM）、电化学阻抗谱（EIS）等技术对纳米ZnO/ITO电极和BSA/r-IgGs/Nano-ZnO/ITO免疫电极进行了表征。BSA/r-IgGs/Nano-ZnO/ITO免疫电极的电化学阻抗响应与OTA浓度的线性关系为0.006-0.01 nM/dm（3），检测限为0.006 nM/dm（3），响应时间为25 s，灵敏度为189 Ω/nM/dm（3）cm（-2），回归系数为0.997。(c)2009爱思唯尔有限公司版权所有。
Nanostructured zinc oxide (Nano-ZnO) film has been deposited onto indium-tin-oxide (ITO) glass plate for co-immobilization of rabbit-immunoglubin antibodies (r-IgGs) and bovine serum albumin (BSA) for ochratoxin-A (OTA) detection. The results of X-ray diffraction (XRD) studies reveal the formation of Nano-ZnO with average particle size as similar to 5.0 nm. Fourier transform infrared (FTIR) spectroscopy, scanning electron microscopy (SEM), electrochemical impedance spectroscopy (EIS) techniques have been used to characterize Nano-ZnO/ITO electrode and BSA/r-IgGs/Nano-ZnO/ITO immunoelectrode. Electrochemical impedimetric response of BSA/r-IgGs/Nano-ZnO/ITO immunoelectrode obtained as a function of OTA concentration exhibits linearity as 0.006-0.01 nM/dm(3), detection limit of 0.006 nM/dm(3), response time as 25 s and sensitivity of 189 Omega/nM/dm(3) cm(-2) with a regression coefficient of 0.997. (c) 2009 Elsevier B.V. All rights reserved.