The toxicity and enzyme activity of a chlorine and sulfate containing aeruginosin isolated from a non-microcystin-producing Planktothrix strain
The toxicity and enzyme activity of a chlorine and sulfate containing aeruginosin isolated from a non-microcystin-producing Planktothrix strain
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DOI：
10.1016/j.hal.2014.07.003
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发表时间：
2014-10-01
期刊：
影响因子：
6.6
通讯作者：
Blom, Judith F.
中科院分区：
文献类型：
--
作者：
Kohler, Esther;Grundler, Verena;Blom, Judith F.
用甲壳类动物阔尾藻（Thamnocephalus platyurus）进行急性毒性试验，研究了6种不同的浮游发藻菌株的毒性。在两个菌株的毒性的存在下，可以解释的微囊藻毒素的发生。另外4株浮游发藻由于微囊藻毒素合成酶（mcy）基因簇的不同突变而不能产生微囊藻毒素。在这些菌株中，毒性归因于含氯和硫酸盐的化合物的存在。在浮游发菌属菌株91/1中分离出这种化合物的主要代表物，称为aerodiosin 828 A，通过2D NMR和MS方法的结构解析揭示了苯乳酸（Pla）、氯亮氨酸（Cleu）、2-羧基-6-（4 '-磺基-木糖基）-八氢吲哚（Choi）和3-氨乙基-1-N-脒基-Δ，-3-吡咯啉（Aeap）残基的存在。发现铜绿菌素828 A对T.对扁尾鱼的LC 50值为22.4 μ M，仅略高于微囊藻毒素的毒性。此外，已测定了Aeroprosin 828 A对凝血酶（IC 50 = 21.8 nM）和胰蛋白酶（IC 50 = 112 nM）的非常强效的抑制值。这些数据支持的假设，含有氯和硫酸基团，这是发现在微囊藻毒素缺陷的浮游发藻株，可以被认为是另一类毒素。(C)2014爱思唯尔有限公司版权所有。
The toxicity of six different Planktothrix strains was examined in acute toxicity assays with the crustacean Thamnocephalus platyurus. The presence of toxicity in two strains could be explained by the occurrence of microcystins. The other four Planktothrix strains were not able to produce microcystins due to different mutations in the microcystin synthetase (mcy) gene cluster. In these strains, toxicity was attributed to the presence of chlorine and sulfate containing compounds. The main representative, called aeruginosin 828A, of such a compound in the Planktothrix strain 91/1 was isolated, and structure elucidation by 2D NMR and MS methods revealed the presence of phenyllactic acid (Pla), chloroleucine (Cleu), 2-carboxy-6-(4'-sulfo-xylosyl)-octahydroindole (Choi), and 3-aminoethyl-1-N-amidino-Delta,-3-pyrroline (Aeap) residues. Aeruginosin 828A was found to be toxic for T. platyurus with a LC50 value of 22.4 mu M, which is only slightly higher than the toxicity found for microcystins. Additionally, very potent inhibition values for thrombin (IC50 = 21.8 nM) and for trypsin (IC50 = 112 nM) have been determined for aeruginosin 828A. These data support the hypothesis that aeruginosins containing chlorine and sulfate groups, which were found in microcystin-deficient Planktothrix strains, can be considered as another class of toxins. (C) 2014 Elsevier B.V. All rights reserved.