Retrieval of lysosomal membrane and acid phosphatase from phagolysosomes of Paramecium caudatum
Retrieval of lysosomal membrane and acid phosphatase from phagolysosomes of Paramecium caudatum
复制标题
尾草履虫吞噬溶酶体溶酶体膜和酸性磷酸酶的回收
作者：
R. Allen;A. Fok
对吞噬细胞中溶酶体膜的命运知之甚少。由于草履虫的消化泡的年龄可以很容易地确定，我们已经能够研究的动态膜事件在老空泡。在吞噬溶酶体阶段（DV-III）的后期，液泡膜经历了小管形成的爆发。小管扩张成囊泡，在薄片和冷冻断裂复制品中具有类似溶酶体的特征。当小管由酸性磷酸酶反应性空泡产生时，也含有酸性磷酸酶活性。我们的结论是，在主动消化后，溶酶体膜连同一些膜相关水解酶一起被全部或部分沿着回收。这些结果的一个逻辑延伸是，溶酶体样囊泡，在通过与初级溶酶体融合而被水解酶重新充电后，被回收回DV-II以供重复使用。
Little is known about the fate of lysosomal membrane in phagocytic cells. Because the age of the digestive vacuoles in Paramecium caudatum can be easily determined, we have been able to study the dynamic membrane events in the older vacuoles. Late in the phagolysosomal stage (DV-III) the vacuole membrane undergoes a burst of tubule formation. The tubules expand into vesicles which have characteristics resembling lysosomes in both thin sections and freeze-fracture replicas. The tubules also contain acid phosphatase activity when they arise from acid phosphatase-reactive vacuoles. We conclude that after active digestion lysosomal membrane is retrieved in whole or in part along with some membrane-associated hydrolases. A logical extension of these results is that the lysosome-like vesicles, after being recharged with hydrolases by fusing with primary lysosomes, are recycled back to DV-II for reuse.
细胞内膜运输：通路、载体和分选装置。
影响因子：
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作者：
Farquhar,MG
通讯作者：
Farquhar,MG