One-Step Hydrothermal Synthesis of Nitrogen-Doped Conjugated Carbonized Polymer Dots with 31% Efficient Red Emission for In Vivo Imaging
One-Step Hydrothermal Synthesis of Nitrogen-Doped Conjugated Carbonized Polymer Dots with 31% Efficient Red Emission for In Vivo Imaging
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一步%20水热%20合成%20of%20氮掺杂%20共轭%20碳化%20聚合物%20点%20与%2031%%20高效%20红色%20发射%20用于%20In%20Vivo%20成像
DOI：
10.1002/smll.201703919
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发表时间：
2018-04-12
期刊：
影响因子：
13.3
通讯作者：
Yang, Bai
中科院分区：
文献类型：
--
作者：
Liu, JunJun;Li, Daowei;Yang, Bai
具有长波长发射、高量子产率（QY）和良好生物相容性的碳点对于生物医学应用来说是非常理想的。在此，报道了一种高效的红色发射氮掺杂碳化聚合物点（CPD）的绿色、简便的水热合成方法，其最佳发射波长为 630 nm 左右。红色发射的 CPD 具有多种优异的性能，包括优异的水分散性、良好的生物相容性、窄带宽发射、与激发无关的发射以及高 QY（10.83%（在水中）和 31.54%（在乙醇中））。进一步的研究证明，如此强烈的红色荧光归因于 CPD 的高效共轭芳香系统和氢键。并且可以通过调节反应前HNO3的用量来调节CPD的荧光性质。此外，所制备的 CPD 已成功用作体外和体内生物成像的荧光探针。更重要的是，生物分布结果表明，大多数CPD及其代谢物不仅通过尿液排泄，而且还通过肝胆系统快速排泄。此外，CPD可以轻松穿过血脑屏障，这可能为通过实时跟踪进行某些脑部疾病的治疗诊断提供有价值的策略。
Carbon dots with long-wavelength emissions, high quantum yield (QY) and good biocompatibility are highly desirable for biomedical applications. Herein, a green, facile hydrothermal synthesis of highly efficient red emissive nitrogen-doped carbonized polymer dots (CPDs) with optimal emission at around 630 nm are reported. The red emissive CPDs possess a variety of superior properties including excellent water dispersibility, good biocompatibility, narrow bandwidth emission, an excitation-independent emission, and high QY (10.83% (in water) and 31.54% (in ethanol)). Further studies prove that such strong red fluorescence is ascribed to the efficient conjugated aromatic systems and hydrogen bonds of CPDs. And the fluorescence properties of CPDs can be regulated by adjusting the dosage of HNO3 before the reaction. Additionally, the as-prepared CPDs are successfully used as a fluorescent probe for bioimaging, both in vitro and in vivo. More importantly, biodistribution results demonstrate that most CPDs and their metabolites are not only excreted in urine but also excreted by hepatobiliary system in a rapid manner. Besides, the CPDs could easily cross the blood brain barrier, which may provide a valuable strategy for the theranostics of some brain diseases through real-time tracking.