Response of soil phoD phosphatase gene to long-term combined applications of chemical fertilizers and organic materials
Response of soil phoD phosphatase gene to long-term combined applications of chemical fertilizers and organic materials
复制标题
土壤phoD磷酸酶基因对化肥与有机肥长期配施的响应
DOI：
10.1016/j.apsoil.2017.06.019
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发表时间：
2017-10-01
影响因子：
4.8
通讯作者：
Chen, Lijun
中科院分区：
文献类型：
--
作者：
Chen, Xiaodong;Jiang, Nan;Chen, Lijun
细菌pHOD基因编码碱性磷酸酶（ALP），这是一种参与土壤中有机磷（P）水解的酶，因此对全球P周期很重要。但是，PHOD基因群落如何对化学肥料和有机材料的长期合并应用做出反应。因此，进行了一项长期（25年）实验，以比较无处理（对照）与化肥（NPK）的影响（对照），玉米稻草（NPK）的化肥和猪粪（NPKM）的化肥对使用高通量测序的PHOD基因社区。还评估了ALP，P形式和其他土壤特性之间的关系。与对照组相比，单独或与玉米稻草结合使用化学肥料的pHOD基因丰度和多样性显着降低，而在化肥中观察到具有相似的多样性和显着增加的丰度。 PHOD基因群落组成分为三组，在规范相关分析（CCOA）图中，并显示出与ALP的部分显着相关性。我们的结果表明，PHOD基因群体的丰度和组成与P储备和P的可用性显着相关。长期施肥重塑了PHOD社区，不同的成员受不同土壤特性的影响，并受到各种肥料的处理。
The bacterial phoD gene encodes alkaline phosphatase (AlP), an enzyme which participates in the hydrolysis of organic phosphorus (P) in soils and is therefore important to the global P cycle. However, how phoD gene communities respond to long-term combined applications of chemical fertilizers and organic materials remains largely unknown. Accordingly, a long-term (25 years) experiment was performed to compare the effects of no treatment (Control) with chemical fertilizers (NPK), chemical fertilizers with corn straw (NPKS), and chemical fertilizers with swine manure (NPKM) on the phoD gene community using high-throughput sequencing. Relationships among AlP, P forms and other soil properties were also evaluated. The phoD gene abundance and diversity significantly decreased with chemical fertilizer alone or in combination with corn straw addition compared to the control, while a similar diversity and significant increased abundance was observed in chemical fertilizer with swine manure. The phoD gene community composition was distributed into three groups in a canonical correlation analysis (CCoA) plot and showed partially significant correlation with AlP. Our results indicate that the abundance and composition of the phoD gene community were significantly correlated with P reserves and P availability. Long-term fertilization reshaped the phoD community, with different members being influenced by different soil properties accompanied by various fertilizer treatments.