Release of a dynorphin A (1-8) degrading enzyme from the spinal cord by intraventricular morphine in the rat.
Release of a dynorphin A (1-8) degrading enzyme from the spinal cord by intraventricular morphine in the rat.
复制标题
大鼠脑室内吗啡从脊髓释放强啡肽 A (1-8) 降解酶。
DOI：
10.1016/0024-3205(88)90222-6
复制
发表时间：
1988
期刊：
影响因子：
6.1
通讯作者：
Tseng,LF
中科院分区：
文献类型：
--
作者：
Tseng,LF
脑室内注射硫酸吗啡（40 μg），从脊髓释放一种酶进入灌注液，降解氨基甲酸乙酯麻醉大鼠的肌啡肽A(1-8)，并在较小程度上降解肌啡肽A（1-13）。该酶不降解肌啡肽A（1-17）、met -脑啡肽、leu -脑啡肽、P物质和神经紧张素。这种dynorphin A（1-8）降解酶被抑肽蛋白、硫硫肽和杆菌肽抑制，在较小程度上被杆菌肽抑制，但不被贝司他汀抑制。dynorphin A（1-8）和抑蛋白蛋白与该酶相互作用的动力学研究表明其本质上是竞争性的。这些发现的药理意义尚不清楚。
Intraventricular injection of morphine sulfate, 40 μg, released an enzyme from the spinal cord into the perfusate which degraded dynorphin A (1–8) and, to a lesser extent, dynorphin A (1–13) in urethane anesthetized rats. The enzyme did not degrade dynorphin A (1–17), Met-enkephalin, Leu-enkephalin, substance P and neurotensin. This dynorphin A (1–8) degrading enzyme was inhibited by aprotinin, thiorphan, and, to a lesser extent, by bacitracin but was not inhibited by bestatin. A kinetic study of the interaction between dynorphin A (1–8) and aprotinin with the enzyme indicated that it is competitive in nature. The pharmacological significance of the findings is still unknown.