Microbial characteristics of methanogenic sludge consortia developed in thermophilic U ASB reactors
Microbial characteristics of methanogenic sludge consortia developed in thermophilic U ASB reactors
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高温 U ASB 反应器中产甲烷污泥群落的微生物特性
DOI：
10.1007/bf00205070
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发表时间：
1993
影响因子：
5
通讯作者：
H. Harada
中科院分区：
文献类型：
--
作者：
S. Uemura;H. Harada
在55℃(运行1)和65℃(运行2)两种不同温度下，研究了温度对高温上流式厌氧污泥床(UASB)中厌氧污泥颗粒化和微生物相互作用的影响。每一次运行包括两个阶段。阶段1通过饲喂醋酸盐进行，为期200天。在第二阶段，两个反应堆都被喂入了醋酸盐和蔗糖的混合物，再持续100天。在第一阶段，两次运行的污泥中都没有出现颗粒化。显微镜观察表明，优势产甲烷菌为甲烷硫杆菌RUN 1，而RUN 2中有大量类甲烷细菌存在。在55~65℃温度范围内，两种污泥利用乙酸盐的产甲烷活性均随温度的升高而增加。由于乙酸酯生长的污泥的产氢活性远高于乙酸盐的产甲烷活性，这表明在高温厌氧污泥中，乙酸酯氧化细菌与嗜水产甲烷菌的共养组合对乙酸盐的整体消除起到了相当大的作用。在阶段2中，分别在运行1和运行2中成功地建立了包被丝状细菌或球菌类型的细菌(两者都可能是产酸细菌)的颗粒。由于颗粒污泥利用醋酸盐的产甲烷活性是醋酸盐培养污泥(第一阶段)的四到五倍，这些“包衣细菌”的共存似乎有助于将醋酸盐消费者封闭在颗粒内。
The effect of temperature on granulation and microbial interaction of anaerobic sludges grown in thermophilic upflow anaerobic sludge bed (UASB) reactors was investigated at two different temperatures, 55°C (Run 1) and 65°C (Run 2). Each run consisted of two phases. Phase 1 was conducted by feeding acetate for a period of 200 days. In Phase 2, both reactors were fed a mixture of acetate and sucrose for a further 100 days. During Phase 1, no granulation occurred in the sludge of either run. Microscopic observation revealed that the predominant methanogen wasMethanothrixin Run 1, whereasMethanobacterium-like bacteria existed to a significant extent in Run 2. The acetate-utilizing methanogenic activity of both sludges increased with increasing test temperature in the range 55–65°C. Since the acetate-grown sludges exhibited far higher H2-utilizing methanogenic activity than acetate-utilizing methanogenic activity, it is suggested that a syntrophic association of acetate-oxidizing bacteria with hydrogenotrophic methanogens was responsible for a considerable portion of the overall acetate elimination in thermophilic anaerobic sludge. During Phase 2, granules coated with either filamentous bacteria or cocci-type bacteria (both presumably acid-forming bacteria) were successfully established in Run 1 and Run 2, respectively. Since the acetate-utilizing methanogenic activities of the granular sludges were four to five times higher than those of the acetate-grown sludges (Phase 1), the co-existence of these “coating bacteria” appeared to contribute to the enclosing of acetate consumers inside granules.