Salt adaptation requires efficient fine-tuning of jasmonate signalling
Salt adaptation requires efficient fine-tuning of jasmonate signalling
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DOI：
10.1007/s00709-013-0591-y
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发表时间：
2014-07-01
期刊：
影响因子：
2.9
通讯作者：
Nick, Peter
中科院分区：
文献类型：
--
作者：
Ismail, Ahmed;Seo, Mitsunori;Nick, Peter
了解植物对盐胁迫的感知、信号和响应机制是植物生物学家的一大兴趣。在应激信号传导中，通常相同的分子参与损伤相关和适应性事件。为了剖析这种复杂性，我们比较了两个葡萄藤细胞系的耐盐性不同的盐度反应。我们遵循快速变化的细胞内容的钠和钙，质外体碱化和较慢的反应水平的茉莉酸，其活性异亮氨酸共轭物和脱落酸，以及芪。镧系元素Gd或外源性钙的时间和敏感性的差异提供了证据早期钠吸收的自适应作用，通过非选择性阳离子通道上游的Ca2+和H+通量。我们发现盐敏感性与不受约束的茉莉酸（JA）信号传导的相关性，而盐适应与茉莉酸及其异亮氨酸共轭物的严格控制相关，伴随着脱落酸的积累和抑制芪类物质，触发防御相关的细胞死亡。这些数据通过一个模型进行了讨论，在该模型中，JA信号的有效微调决定了细胞是否会朝着适应或程序细胞死亡的方向发展。
Understanding the mechanism by which plants sense, signal and respond to salinity stress is of great interest to plant biologists. In stress signalling, often the same molecules are involved in both damage-related and adaptive events. To dissect this complexity, we compared the salinity responses of two grapevine cell lines differing in their salinity tolerance. We followed rapid changes in the cellular content of sodium and calcium, apoplastic alkalinisation and slower responses in the levels of jasmonic acid, its active isoleucine conjugate and abscisic acid, as well as of stilbenes. Differences in timing and sensitivity to either the lanthanoid Gd or exogenous calcium provide evidence for an adaptive role of early sodium uptake through non-selective cation channels acting upstream of Ca2+ and H+ fluxes. We find a correlation of salt sensitivity with unconstrained jasmonate (JA) signalling, whereas salt adaptation correlates with tight control of jasmonic acid and its isoleucine conjugate, accompanied by accumulation of abscisic acid and suppression of stilbenes that trigger defence-related cell death. The data are discussed by a model where efficient fine-tuning of JA signalling determines whether cells will progress towards adaptation or programme cell death.