Oral Candida infection and colonization in solid organ transplant recipients
Oral Candida infection and colonization in solid organ transplant recipients
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DOI：
10.1111/j.1399-302x.2009.00505.x
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发表时间：
2009-06-01
影响因子：
--
通讯作者：
Burleson, J.
中科院分区：
文献类型：
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作者：
Dongari-Bagtzoglou, A.;Dwivedi, P.;Burleson, J.
据报道，口腔念珠菌携带和感染与移植受者发生全身感染的更大风险相关；然而，以前没有对口腔念珠菌滴度和种类进行系统分析。本研究的目的是确定该人群口咽念珠菌病的患病率、口腔携带者状况、念珠菌滴度和种类。包括90名肾脏和心脏移植患者和72名年龄匹配的健康对照。将口腔黏膜拭子和标准量的未刺激唾液涂于Chromagar （TM）念珠菌上，计算每毫升菌落形成单位。最初的物种形成以菌落颜色为基础，并通过标准胚管、生物分型或聚合酶链反应测定来证实。在7例移植患者中检测到白色念珠菌感染，对照组中无一例。移植组口腔念珠菌滴度明显高于对照组。免疫抑制剂的剂量或类型与口腔念珠菌滴度或感染之间没有统计学意义的关系。与对照受试者相比，移植受试者被一个以上物种定植的比例明显更高。移植对象中最常见的菌种组合是白色念珠菌和心念珠菌。13.5%的移植物携带者中分离到光棘球蚴，对照组中没有分离到。在移植人群中发现口腔念珠菌感染和携带滴度增加。虽然大多数移植患者是由白色念珠菌定植的，但光滑念珠菌似乎是第二常见的物种。
Oral Candida carriage and infection have been reported to be associated with a greater risk for systemic infection in transplant recipients; however, a systematic analysis of the oral Candida titers and species has not been previously conducted. The objectives of this study were to determine the prevalence of oropharyngeal candidiasis, the oral carrier status, Candida titers and species in this population.Ninety kidney and heart transplant subjects and 72 age-matched healthy controls were included. Swabs from the oral mucosa and a standardized amount of unstimulated saliva were plated on Chromagar (TM) Candida, and colony-forming units per millilitre were calculated. Initial speciation was based on colony color and was confirmed by standard germ tube, biotyping, or polymerase chain reaction assays.Infection with C. albicans was detected in seven transplant subjects and none of the controls. The transplant group had significantly higher oral Candida titers than the control group. There were no statistically significant relationships between the dose or type of immunosuppressants and oral Candida titers or infection. A significantly higher percentage of transplant subjects were colonized by more than one species, compared with control subjects. The most frequent species combination in transplant subjects was C. albicans and C. glabrata. C. glabrata was isolated from 13.5% of transplant carriers and none of the controls.Increased oral Candida infection and carriage titers were found in the transplant population. Although the majority of transplant patients were colonized by C. albicans, C. glabrata appears to emerge as the second most prevalent species.