Cytokine secretion in decidual mononuclear cells from term human pregnancy with or without labour:: ELISPOT detection of IFN-γ, IL-4, IL-10, TGF-β and TNF-α
Cytokine secretion in decidual mononuclear cells from term human pregnancy with or without labour:: ELISPOT detection of IFN-γ, IL-4, IL-10, TGF-β and TNF-α
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DOI：
10.1016/j.jri.2005.12.009
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发表时间：
2006-08-01
影响因子：
3.4
通讯作者：
Ernerudh, J.
中科院分区：
文献类型：
--
作者：
Gustafsson, C.;Hummerdal, P.;Ernerudh, J.
细胞因子被认为在维持妊娠和人类引产过程中起着重要作用。为探讨临产前后蜕膜组织中细胞因子干扰素-γ(干扰素-γ)、白介素4(IL-4)、白介素10(IL-10)、转化生长因子-β(TGF-β)和肿瘤坏死因子-α(TNF-α)的分泌情况，对32例择期剖宫产孕妇(n=17)和正常阴道分娩后(n=15)健康妇女的蜕膜组织进行检测。用ELISPOT分析单个核细胞分泌细胞因子的情况。为了验证广泛使用的剖宫产和阴道分娩后胎盘组织作为临产前和产后代表的方法，收集了12例健康妇女的胎盘组织，研究了前列腺素途径酶环氧合酶-2(COX-2)和微粒体前列腺素E-2合成酶(MPGES)的表达。足月妊娠人单个核细胞自发分泌干扰素-γ、白介素4、白介素10、转化生长因子-β和肿瘤坏死因子-α。分娩前后细胞因子分泌无明显差异，提示蜕膜白细胞不是终止妊娠过程中细胞因子调节的主要细胞群。阴道分娩后的胎盘组织中前列腺素调节分子COX-2和mPGES的mRNA表达高于临产前的剖宫产组织，证实该模型可作为临产前后状态的代表。(C)2006爱思唯尔爱尔兰有限公司。保留所有权利。
Cytokines are believed to be important in maintaining pregnancy and in the process of labour induction in humans. The aim of this study was to investigate the secretion of the cytokines interferon-gamma (IFN-gamma), interleukin-4 (IL-4), LL-10, transforming growth factor-beta (TGF-beta) and tumour necrosis factor-alpha (TNF-alpha) in decidual tissue with or without labour.Decidual tissue was collected from 32 healthy women undergoing elective caesarean sections before the onset of labour (n = 17) or after normal vaginal delivery (n = 15). Mononuclear cells were analysed for cytokine secretion with ELISPOT. To validate the widely used method of tissue collected at caesarean sections and after vaginal deliveries as a representative of before and after labour, respectively, placenta biopsies were collected from 12 healthy women to study the expression of the prostaglandin pathway enzymes cyclooxygenase-2 (COX-2) and microsomal prostaglandin E-2 synthase (mPGES).Decidual mononuclear cells from term human pregnancy spontaneously secrete IFN-gamma, IL-4, IL- 10, TGF-beta and TNF-alpha. No difference was seen in cytokine secretion with or without labour, indicating that decidual leukocytes are not the main cell population responsible for plausible cytokine regulation in the process of termination of pregnancy. Placental tissues obtained after vaginal delivery showed a higher mRNA expression of the prostaglandin regulating molecules COX-2 and mPGES than tissues from caesarean sectioris before the onset of labour, validating that the model can be used as a representative of the state before and after labour. (c) 2006 Elsevier Ireland Ltd. All rights reserved.