INHERENT LIMITATIONS OF NONDESTRUCTIVE CHLOROPHYLL METERS - A COMPARISON OF 2 TYPES OF METERS
INHERENT LIMITATIONS OF NONDESTRUCTIVE CHLOROPHYLL METERS - A COMPARISON OF 2 TYPES OF METERS
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DOI：
10.21273/hortsci.27.1.69
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发表时间：
1992-01-01
期刊：
影响因子：
1.9
通讯作者：
BUGBEE, B
中科院分区：
文献类型：
--
作者：
MONJE, OA;BUGBEE, B
对两种非破坏性叶绿素仪与一种标准的破坏性叶绿素测量技术进行了比较。非破坏性叶绿素计是1)定制的单波长计，以及2)最近推出的美能达双波长叶绿素计（型号SPAD-502）。对于叶绿素浓度在100 - 600 mg.m-2之间的叶片，这两种测量仪的数据与叶绿素的破坏性测量值密切相关（r2分别为0.90和0.93），但这两种测量仪一致高估了超出该范围的叶绿素。虽然双波长计的准确度略高于单波长计（r2更高），但叶片细胞的光散射特性和叶片中叶绿素的不均匀分布似乎限制了所有波长计估计体内叶绿素浓度的能力。
Two types of nondestructive chlorophyll meters were compared with a standard, destructive chlorophyll measurement technique. The nondestructive chlorophyll meters were 1) a custom built, single-wavelength meter, and 2) the recently introduced, dual-wavelength, chlorophyll meter from Minolta (model SPAD-502). Data from both meters were closely correlated with destructive measurements of chlorophyll (r2 = 0.90 and 0.93; respectively) for leaves with chlorophyll concentrations ranging from 100 to 600 mg.m-2, but both meters consistently overestimated chlorophyll outside this range. Although the dual-wavelength meter was slightly more accurate than the single-wavelength meter (higher r2), the light-scattering properties of leaf cells and the nonhomogeneous distribution of chlorophyll in leaves appear to limit the ability of all meters to estimate in vivo chlorophyll concentration.