Design and preparation of quantum dots fluorescent probes for in situ identification of Microthrix parvicella in bulking sludge
Design and preparation of quantum dots fluorescent probes for in situ identification of Microthrix parvicella in bulking sludge
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DOI：
10.1007/s00253-015-7015-1
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发表时间：
2016-01-01
影响因子：
5
通讯作者：
Jia, Guozhi
中科院分区：
文献类型：
--
作者：
Fei, Xuening;Sun, Wenke;Jia, Guozhi
采用量子点荧光探针原位检测微小微丝菌（Microthrix parvicella，M. parvicella）在污泥膨胀中的作用。在CdTe/CdS量子点的制备过程中，以11-巯基十一酸（11-acid）和16-巯基十六烷酸（16-acid）为稳定剂。通过红外光谱（FT-IR）、X射线衍射（XRD）和透射电镜（TEM）对所制备的量子点探针进行表征，结果表明：CdTe/CdS量子点形成了核壳结构，长碳链成功接枝到其表面。由于长碳链的抑制作用，三种量子点探针具有不同的结晶度和粒径。光学性质测试结果表明，虽然形成的核壳结构和长碳链影响了量子点探针的荧光强度、吸附和发射光谱，但探针B和C具有较大的斯托克斯位移，分别为82和101 nm，有利于其荧光标记性能。在M.探针B和C能有效地吸附在M. parvicella通过疏水键连接，进而鉴定出M.它们独特的荧光。此外，发现更好的疏水性导致更好的识别效率。
A series of quantum dots (QDs) fluorescent probes for the in situ identification of Microthrix parvicella (M. parvicella) in bulking sludge were designed and prepared. In the preparation of CdTe/CdS QDs, the 11-mercaptoundecanoic acid (11-acid) and 16-mercaptohexadecanoic acid (16-acid) were used as the stabilizer. The prepared QDs probes were characterized by Fourier transform infrared (FT-IR), X-ray diffraction (XRD), and transmission electron microscopy (TEM), and the results showed that the CdTe/CdS QDs formed a core-shell structure and the long carbon chain was successfully grafted onto its surface. And the three QDs probes had different crystallinity and particle size, which was due to the inhibition effect of long carbon chain. The optical properties test results showed that although the formed core-shell structure and long carbon chain affected the fluorescent intensity, adsorption, and emission spectra of the QDs probes, the probes B and C had a large stokes-shift of 82 and 101 nm, which was a benefit for their fluorescent labeling property. In the fluorescent identification of M. parvicella, the probes B and C effectively adsorbed onto the surface of M. parvicella through a hydrophobic bond, and then identified M. parvicella by their unique fluorescence. In addition, it was found that a better hydrophobic property resulted in better identification efficiency.