HISTOMORPHOMETRY OF THE OPTIC NERVES OF NORMAL DOGS AND DOGS WITH HEREDITARY GLAUCOMA
HISTOMORPHOMETRY OF THE OPTIC NERVES OF NORMAL DOGS AND DOGS WITH HEREDITARY GLAUCOMA
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DOI：
10.1016/s0014-4835(05)80085-5
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发表时间：
1995-01-01
影响因子：
3.4
通讯作者：
GELATT, KN
中科院分区：
文献类型：
--
作者：
BROOKS, DE;STRUBBE, DT;GELATT, KN
遗传性原发性开角型青光眼的beagle犬，与其他动物模型的人类青光眼不同，具有缓慢进行性，持续的眼压升高。我们将三只早期青光眼小猎犬和两只晚期青光眼小猎犬的眼内压升高对视神经轴突的影响与四只年龄匹配的正常狗的视神经进行了比较。取筛板后1 mm的塑料嵌埋视神经横切面（1 μ m），用甲苯胺蓝染色。利用计算机图像分析系统对整个视神经内16个大小相等的横截区域内直径0.2 ~ 2.0 μ m的轴突进行计数和测量。正常犬、早期青光眼犬、晚期青光眼犬视神经轴突平均直径分别为1.53、1.25、1.13 μ m。正常犬视神经轴突总数平均为148 303。每条神经中约有16%的轴突纤维被计数。小猎犬视神经中枢区域直径大于2.0 μ m的视神经轴突数量减少60%。青光眼比格犬外周视神经轴突直径较大，对眼压升高的抵抗能力较强。青光眼小猎犬的轴突数量明显增加，直径0.6 ~ 0.8 μ m。
The beagle dog with hereditary primary open-angle glaucoma, unlike other animal models of human glaucoma, possesses a slowly progressive, sustained elevation of intraocular pressure. The effects of this insidious elevation in intraocular pressure on the axons of the optic nerves of three beagles at early stages of glaucoma and two beagles with advanced signs of glaucoma were compared to the optic nerves of four age-matched normal dogs. Plastic embedded optic nerve cross-sections (1 mu m) 1 mm posterior to the lamina cribrosa were osmicated and stained with Toluidine Blue. Axons from 0.2 to 2.0 mu m in diameter were counted and measured in 16 cross-sectional regions of equal size within the whole optic nerve using a computerized image analysis system. The mean optic nerve axon diameters in the normal, early glaucomatous, and advanced glaucomatous dogs were 1.53, 1.25 and 1.13 mu m respectively. The average total optic nerve axon count in the normal dogs was 148 303. Approximately 16% of the total axonal fibers were counted in each nerve. The counts of optic nerve axons 2.0 mu m or greater in diameter were reduced by up to 60% in the central regions of the optic nerves of affected beagles. The large diameter axons of the peripheral optic nerve of the beagle dogs with glaucoma were more resistant to the elevated intraocular pressure. The counts of axons > 0.6 to 0.8 mu m in diameter were significantly increased in glaucomatous beagles.