Differential Effects of Circulating IgA Isolated from Patients with IgA Nephropathy on Superoxide and Fibronectin Production of Mesangial Cells
Differential Effects of Circulating IgA Isolated from Patients with IgA Nephropathy on Superoxide and Fibronectin Production of Mesangial Cells
复制标题
从 IgA 肾病患者分离的循环 IgA 对系膜细胞超氧化物和纤连蛋白产生的不同影响
作者：
H.C. Chen;J. Guh;J. Chang;Y. Lai
背景：伊加肾病（IgAN）的特征是伊加主要沉积于肾小球系膜区. IgA肾病患者血清伊加水平升高，推测其与系膜病变有关。然而，循环伊加对系膜细胞的直接影响尚不清楚。研究方法：我们研究了从IgAN患者中分离的血清和伊加对培养的大鼠系膜细胞的胸苷摄取、超氧化物和纤维连接蛋白的产生以及纤维连接蛋白mRNA表达的影响，并将结果与从非IgA系膜增生性肾小球肾炎（MsPGN）患者和正常对照中分离的伊加的影响进行了比较。用溴化氰活化的Sepharose 4 B与羊抗人伊加抗血清偶联，用亲和层析法分离伊加。结果如下：我们的研究结果表明，从IgAN患者的血清和伊加剂量依赖性地增加肾小球系膜细胞的有丝分裂测定3 H-标记的胸苷摄取。IgAN患者血清和伊加对胸腺嘧啶核苷的摄取明显高于MsPGN患者和正常人。IgA肾病患者血清和伊加可显著增加肾小球系膜细胞超氧化物歧化酶和纤连蛋白的产生及纤连蛋白mRNA的表达。超氧化物歧化酶和纤维连接蛋白的产生也明显高于MsPGN患者和正常对照组。结论：我们的研究结果表明，从IgAN患者中分离的循环伊加与MsPGN患者和正常对照组不同，可能潜在地诱导IgAN中肾小球系膜细胞的氧化损伤和细胞外基质的产生。
Background: IgA nephropathy (IgAN) is characterized by predominant deposition of IgA in the glomerular mesangium. Serum IgA is often elevated in patients with IgAN, and it has been postulated that it is responsible for the mesangial lesions. However, the direct effect of circulating IgA on mesangial cells is not clear. Methods: We investigated the effects of sera and IgA which were isolated from patients with IgAN on thymidine uptake, superoxide and fibronectin production and fibronectin mRNA expression of cultured rat mesangial cells, and we compared the findings to the effects of IgA isolated from patients with non-IgA mesangial proliferative glomerulonephritis (MsPGN) and normal controls. IgA was isolated with affinity chromatography using cyanogen bromide activated Sepharose 4B coupled to sheep antihuman IgA antiserum. Results: Our results demonstrated that both sera and IgA from patients with IgAN dose-dependently increased mitogenesis of mesangial cells as measured by 3H-labeled thymidine uptake. The thymidine uptake by sera and IgA isolated from patients with IgAN was significantly higher than that of sera and IgA isolated from patients with MsPGN and normal controls. Sera and IgA from patients with IgAN significantly enhanced superoxide and fibronectin production and fibronectin mRNA expression of mesangial cells. The superoxide and fibronectin production was also significantly higher as compared with patients with MsPGN and normal controls. Conclusions: Our results indicate that circulating IgA isolated from patients with IgAN is different from that of patients with MsPGN and normal controls and may potentially induce oxidative injury and production of extracellular matrix of glomerular mesangial cells in IgAN.
IgA 肾病：形态表达和发病机制。
DOI：
10.1016/s0272-6386(12)81011-0
发表时间：
1994
期刊：
American journal of kidney diseases : the official journal of the National Kidney Foundation
影响因子：
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作者：
Emancipator,SN
通讯作者：
Emancipator,SN