Effect of 2-hexadecynoic acid on cultured 7288C hepatoma cells
Effect of 2-hexadecynoic acid on cultured 7288C hepatoma cells
复制标题
2-十六氰酸对培养7288C肝癌细胞的影响
作者：
G. C. Upreti;M. Matocha;R. Wood
研究了2-己烯酸对7288 （HTC）细胞生长和脂质代谢的影响。在低密度和高密度条件下，两种方法测定的LD50为35 ~ 85 μM。通过α-氨基异丁酸盐泄漏测定，生长降低不是由于质膜受损造成的。DNA合成不受乙炔酸的影响，对RNA和蛋白质合成的影响似乎次于对脂质代谢的影响。2-十六酸至少在两个水平上抑制了HTC细胞的脂质代谢。大量研究和细胞和介质脂质中放射性醋酸盐分布的数据表明，脂肪酸延伸和酰化，特别是甘油三酯合成受到抑制。
The effects of 2-hexadecynoic acid on the growth and lipid metabolism of cultured 7288 (HTC) cells have been evaluated. Growth was inhibited by the acetylenic acid: the LD50 was 35–85 μM as determined by two methods at low and high cell densities. Reduced growth did not result from damaged plasma membranes as determined by α-amino isobutyrate leakage. DNA synthesis was unaffected by the acetylenic acid and the effect on RNA and protein synthesis appeared to be secondary to the effects on lipid metabolism. The 2-hexadecynoic acid inhibited lipid metabolism of the HTC cells at least at two levels. Data from both mass studies and radioactive acetate distributions in cellular and media lipids indicated that fatty acid elongation and acylation, especially triglyceride synthesis, were inhibited.