Epstein-Barr virus glycoprotein gp85 associates with the BKRF2 gene product and is incompletely processed as a recombinant protein.
Epstein-Barr virus glycoprotein gp85 associates with the BKRF2 gene product and is incompletely processed as a recombinant protein.
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DOI：
10.1006/viro.1993.1388
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发表时间：
1993-08
期刊：
影响因子：
3.7
通讯作者：
L. R. Yaswen;E. Stephens;L. Davenport;L. Hutt-Fletcher
中科院分区：
文献类型：
--
作者：
L. R. Yaswen;E. Stephens;L. Davenport;L. Hutt-Fletcher
爱泼斯坦-巴尔病毒（EB病毒）糖蛋白gp 85是EB病毒gH同源物，被认为参与病毒穿透B细胞膜。然而，尽管糖蛋白在功能上是重要的，但在感染的细胞和病毒体中发现其丰度非常低。为了便于分析gp 85的结构和功能，构建了表达gp 85的重组痘苗病毒。重组gp 85被从gp 85序列衍生的肽制备的多克隆抗体识别，但不被与天然分子反应的单克隆抗体识别。与天然gp 85不同，重组蛋白不含对糖苷内切酶H具有抗性的糖，并且不转运至细胞表面。发现天然蛋白质与另外两种表观M（r）为25，000和42，000的糖蛋白相关。针对源自BKRF 2开放阅读框中序列的肽制备的抗体免疫沉淀迁移率为gp 85及其相关25，000 Da分子的糖蛋白。这些数据表明，BKRF 2基因产物，如由其位置同源物gL的单纯疱疹病毒和人巨细胞病毒的UL 115基因产物编码的，与gp 85相关，并可能是糖蛋白加工所需的。
The Epstein-Barr virus (EBV) glycoprotein gp85 is the EBV gH homologue and is thought to be involved in penetration of virus through the B-cell membrane. However, although the glycoprotein is functionally important, it is found in very low abundance in infected cells and in the virion. To facilitate analysis of the structure and function of gp85, recombinant vaccinia viruses were constructed to express the glycoprotein. Recombinant gp85 was recognized by polyclonal antibody made to a peptide derived from the gp85 sequence, but not by monoclonal antibodies that reacted with the native molecule. Unlike native gp85, the recombinant protein contained no sugars that were resistant to endoglycosidase H and it was not transported to the cell surface. The native protein was found to be associated with two additional glycoproteins with apparent M(r) of 25,000 and 42,000. Antibody made to a peptide derived from a sequence in the BKRF2 open reading frame immunoprecipitated glycoproteins with the mobilities of gp85 and its associated 25,000-Da molecule. These data suggest that the BKRF2 gene product, like that encoded by its positional homologues gL of herpes simplex virus and the UL115 gene product of human cytomegalovirus, associates with gp85 and may be required for glycoprotein processing.