An HLA-C-specific DNA probe
An HLA-C-specific DNA probe
复制标题
HLA-C 特异性 DNA 探针
作者：
T. Strachan;A. Dodge;D. Smillie;P. Dyer;R. Sodoyer;B. Jordan;Rodney Harris
对I类HLA基因的可用核苷酸序列数据的分析已经确定，第七内含子是表达最高程度的基因座特异性（非等位基因之间的序列差异百分比减去等位基因之间的序列差异百分比）的基因区域之一。我们已经从HLA-Cw 3基因亚克隆了包括该区域的短DNA序列。通过将两个克隆pC 250和pC 800与一组含有I类HLA基因的克隆在高严格条件下杂交来测试它们。在允许与C-位点基因的强杂交信号的条件下，pC 800也表达与其它I类基因的弱但显著的杂交，而pC 250似乎仅与C-位点基因杂交。杂交的pC 250探针在高严格的Hind III消化的基因组DNA从一组无关的个人和纯合分型细胞系显示在所有情况下的一个单一的带。然而，对Eco RI消化的DNA的等效杂交显示了两个杂交带，一个在7.9 kb处，其与血清学上定义的Cw 5和Cw 8等位基因相关，另一个在7.6 kb处，其与Cw 1、Cw 2、Cw 3、Cw 4、Cw 6和Cw 7等位基因相关。
Analysis of available nucleotide sequence data for class I HLA genes has established that the seventh intron is one of the gene regions which expresses the highest degree of locus specificity (the percentage sequence divergence between nonallelic genes minus the percentage sequence divergence between allelic genes). We have subcloned short DNA sequences including this region from the HLA-Cw3 gene. Two clones, pC250 and pC800, were tested by hybridizing them at high stringency to a panel of clones containing class I HLA genes. Under conditions permitting a strong hybridization signal with a C-locus gene, pC800 also expressed a weak but significant hybridization to other class I genes, while pC250 appeared to hybridize exclusively to the C-locus gene. Hybridization of the pC250 probe at high stringency to Hind III-digested genomic DNA from a panel of unrelated individuals and homozygous typing cell lines revealed a single band in all cases. However, equivalent hybridization against Eco RI-digested DNA revealed two hybridization bands, one at 7.9 kb which correlated with the serologically defined Cw5 and Cw8 alleles, and one at 7.6 kb which correlated with the Cw1, Cw2, Cw3, Cw4, Cw6, and Cw7 alleles.
HLA-Bw58 的完整一级结构。
DOI：
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发表时间：
1985
期刊：
The Journal of biological chemistry
影响因子：
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作者：
Ways,JP;Coppin,HL;Parham,P
通讯作者：
Parham,P
HLA-A2 基因的克隆和完整序列：在核苷酸水平上分析两个 HLA-A 等位基因。
DOI：
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发表时间：
1985
期刊：
Journal of immunology (Baltimore, Md. : 1950)
影响因子：
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作者：
Koller,BH;Orr,HT
通讯作者：
Orr,HT