Viscous control of cellular respiration by membrane lipid composition
Viscous control of cellular respiration by membrane lipid composition
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DOI：
10.1126/science.aat7925
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发表时间：
2018-12-07
期刊：
影响因子：
56.9
通讯作者：
Keasling, Jay D.
中科院分区：
文献类型：
--
作者：
Budin, Itay;de Rond, Tristan;Keasling, Jay D.
脂质成分决定了生物膜的物理特性，并且在生物体之间和内部可能存在很大差异。我们描述了能量转换膜的粘度在细胞呼吸中的特定作用。大肠杆菌中的脂肪酸生物合成工程使我们能够通过控制不饱和或支化脂质的丰度来滴定 10 倍范围内的内膜粘度。这些流化脂质严格控制呼吸代谢，这种效应可以用电子传递链 (ETC) 的定量模型来解释，该模型以酶和电子载体（醌）之间的扩散耦合反应为特征。脂质不饱和度还调节工程芽殖酵母菌株中的线粒体呼吸。因此，ETC 中的扩散可能成为呼吸膜中脂质组成的进化限制。
Lipid composition determines the physical properties of biological membranes and can vary substantially between and within organisms. We describe a specific role for the viscosity of energy-transducing membranes in cellular respiration. Engineering of fatty acid biosynthesis in Escherichia coil allowed us to titrate inner membrane viscosity across a 10-fold range by controlling the abundance of unsaturated or branched lipids. These fluidizing lipids tightly controlled respiratory metabolism, an effect that can be explained with a quantitative model of the electron transport chain (ETC) that features diffusion-coupled reactions between enzymes and electron carriers (quinones). Lipid unsaturation also modulated mitochondrial respiration in engineered budding yeast strains. Thus, diffusion in the ETC may serve as an evolutionary constraint for lipid composition in respiratory membranes.