Luminescent nanocrystals in phospholipid micelles for bioconjugation: An optical and structural investigation
Luminescent nanocrystals in phospholipid micelles for bioconjugation: An optical and structural investigation
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DOI：
10.1016/j.jcis.2008.06.018
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发表时间：
2008-09-15
影响因子：
9.9
通讯作者：
Curri, Maria Lucia
中科院分区：
文献类型：
--
作者：
Depalo, Nicoletta;Mallardi, Antonia;Curri, Maria Lucia
有机封端的发光CdSe@ZnS纳米晶（NC）已被纳入嵌段共聚物胶束，由聚乙二醇改性的磷脂（PEG脂质）形成。得到的水溶性NC包括PEG脂质胶束已与牛血清白蛋白（BSA）的共轭。整个过程已通过使用光学，结构和电泳互补技术进行了研究。这种综合的方法已经允许阐明关键问题，如时间和温度对PEG脂质/NC聚集体结构的相行为的影响，胶束形成和生物缀合之前和之后的NC的发射性质，以及缀合对生物部分的影响。总体结果提供了关于生物缀合物的制造、它们的稳定性和制备过程再现性的相关见解，鉴于所得蛋白质修饰的NC作为用于制造各种超分子组装体以在生物传感和诊断应用中利用的多功能混合构建块的用途。(C)2008年爱思唯尔公司所有战斗保留。
Organic capped luminescent CdSe@ZnS nanoctystals (NCs) have been incorporated in block copolymer micelles, formed by polyethylene glycol modified phospholipids (PEG lipids). The obtained water soluble NC including PEG lipid micelles have been conjugated with bovine serum albumine (BSA). The entire process has been investigated by using optical, structural and electrophoretic complementary techniques. Such an integrated approach has allowed to elucidate critical issues, such as the time and temperature effects on the phase behavior of the PEG lipid/NC aggregate structures, the emitting properties of the NCs before and after micelle formation and bio-conjugation and the effect of conjugation on the biological moiety. The overall results provide relevant insight on the fabrication of the bio-conjugates, on their stability and on preparative procedure reproducibility, in view of the use of the resulting protein decorated NCs as multifunctional hybrid building blocks for the fabrication of a variety of supramolecular assemblies to exploit in biological sensing and diagnostic applications. (C) 2008 Elsevier Inc. All Fights reserved.