Quantification of cellular autofluorescence of human skin using multiphoton tomography and fluorescence lifetime imaging in two spectral detection channels.
Quantification of cellular autofluorescence of human skin using multiphoton tomography and fluorescence lifetime imaging in two spectral detection channels.
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DOI：
10.1364/boe.2.003295
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发表时间：
2011-12-01
影响因子：
3.4
通讯作者：
Dunsby C
中科院分区：
文献类型：
--
作者：
Patalay R;Talbot C;Alexandrov Y;Munro I;Neil MA;König K;French PM;Chu A;Stamp GW;Dunsby C
我们探索了使用两个光子显微镜和荧光寿命成像（FLIM）在人体皮肤中通过两个光谱检测通道成像内源荧光团的诊断潜力。新鲜切除的良性发育不良痣 (DN) 和恶性结节性基底细胞癌 (nBCC) 在 760 nm 处激发。所产生的荧光信号被手动逐个细胞地分箱。这提高了使用双指数衰减模型拟合的可靠性，并允许识别和研究组织内不同细胞群的荧光特征。我们还对不同诊断组之间进行了直接比较。使用 Mann-Whitney U 检验，nBCC 和 DN 之间的中位平均荧光寿命分别为 2.79 ns 与 2.52 ns（蓝色通道，300-500 nm）和 2.08 ns 与 1.33 ns（绿色通道，500-640 nm）之间存在统计学显着差异（p < 0.01）。还讨论了荧光寿命参数分布和患者间变异性的进一步差异。
We explore the diagnostic potential of imaging endogenous fluorophores using two photon microscopy and fluorescence lifetime imaging (FLIM) in human skin with two spectral detection channels. Freshly excised benign dysplastic nevi (DN) and malignant nodular Basal Cell Carcinomas (nBCCs) were excited at 760 nm. The resulting fluorescence signal was binned manually on a cell by cell basis. This improved the reliability of fitting using a double exponential decay model and allowed the fluorescence signatures from different cell populations within the tissue to be identified and studied. We also performed a direct comparison between different diagnostic groups. A statistically significant difference between the median mean fluorescence lifetime of 2.79 ns versus 2.52 ns (blue channel, 300-500 nm) and 2.08 ns versus 1.33 ns (green channel, 500-640 nm) was found between nBCCs and DN respectively, using the Mann-Whitney U test (p < 0.01). Further differences in the distribution of fluorescence lifetime parameters and inter-patient variability are also discussed.