Age-related settlement success by cyprids of the barnacle Balanus amphitrite, with special reference to consumption of cyprid storage protein
Age-related settlement success by cyprids of the barnacle Balanus amphitrite, with special reference to consumption of cyprid storage protein
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DOI：
10.1007/bf00993652
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发表时间：
1996-11-01
期刊：
影响因子：
2.4
通讯作者：
Fusetani, N
中科院分区：
文献类型：
--
作者：
Satuito, CG;Shimizu, K;Fusetani, N
新的蜕皮（0-d-老）的藤壶藤壶amphitrite达尔文的cyprids被阻止从0到14天，在四个不同的温度（25，20，15和5摄氏度的治疗）解决。使用腈基纤维素膜测定法评价了延长Cyprid寿命对沉降成功的影响。此外，蛋白质提取物从这些Cyprids制备进行了分析，使用凝胶电泳，以表征年龄对蛋白质含量和组成的影响。在25 ℃（P < 0.001）、20 ℃（P < 0.001）和15 ℃（P < 0.05）下，幼虫的定居成功率受到显著影响，而在5 ℃下，年龄组之间的定居成功率差异可以忽略不计（P = 0.09）。Cyprids的定居成功率随着时间的增加而增加，最多持续3 d（P < 0.001，阶段I），随后定居成功率显著下降（P < 0.001，阶段II）。温度对第I阶段的沉降没有显著影响（P = 0.17，第I阶段），随后沉降成功率显著下降（P < 0.001，第II阶段）。在第一阶段，温度对沉降没有显著影响（P = 0.17），但在第二阶段，随着年龄的增长，沉降成功率确实有所下降（p < 0.001）。凝胶电泳显示，随着年龄的增加，在25，20和15摄氏度的Cyprid存储蛋白CMP（Cyprid主要蛋白质）的数量显着下降，但CMP水平保持不变，在5摄氏度。这些结果表明，蜕皮后的Cyprid阶段，幼虫可能仍然需要一个解决能力的实现期。这可能是通过CMP利用在第一阶段，耗尽其在第二阶段可能是负责减少解决成功与塞浦路斯年龄，使剩余的CMP商店不能再支持成人结构的生产后解决。
Newly molted (0-d-old) cyprids of the barnacle Balanus amphitrite Darwin were prevented from settling for 0 to 14 d at four different temperatures (25, 20, 15 and 5 degrees C treatments). The effect on settlement success of prolonging the cyprid lifetime was evaluated using a nitrilocellulose membrane assay. In addition, protein extract prepared from these cyprids was analyzed using gel electrophoresis to characterize the effect of age on protein content and composition. Settlement success was significantly affected for larvae aged at 25 (P < 0.001), 20 (P < 0.001) and 15 degrees C (P < 0.05), while differences in settlement success between age groups was negligible at 5 degrees C (P = 0.09). Settlement success of cyprids increased with time for up to 3 d (P < 0.001, Phase I), following which settlement success significantly declined (P < 0.001, Phase II). Temperature had no significant effect on settlement in Phase I (P = 0.17), Phase I), following which settlement success significantly declined (P < 0.001, Phase II). Temperature had no significant effect on settlement in Phase I (P = 0.17), but did enhance the decline in settlement success with age during Phase II (p < 0.001). Gel electrophoresis revealed a significant decline in the quantity of the cyprid storage protein CMP (Cyprid Major Protein) with increasing age at 25, 20 and 15 degrees C, but CMP levels remained constant at 5 degrees C. These results suggest that, upon molting to the cyprid stage, larvae may still require a settlement-competence attainment period. This may be achieved by CMP utilization during Phase I, depletion of which during Phase II may be responsible for reduction in settlement success with cyprid age such that remaining CMP stores can no longer support the production of adult structures following settlement.