Effect of the Short-Term Use of Fluoroquinolone and β-Lactam Antibiotics on Mouse Gut Microbiota.
Effect of the Short-Term Use of Fluoroquinolone and β-Lactam Antibiotics on Mouse Gut Microbiota.
复制标题
短期使用氟喹诺酮和β-内酰胺抗生素对小鼠肠道微生物群的影响
DOI：
10.2147/idr.s281274
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发表时间：
2020
影响因子：
3.9
通讯作者：
Tang LL
中科院分区：
文献类型：
--
作者：
Gu SL;Gong Y;Zhang J;Chen Y;Wu Z;Xu Q;Fang Y;Wang J;Tang LL
背景抗生素在感染性疾病的治疗中发挥着重要作用。然而，抗生素的过度使用增加了耐药细菌的传播，并导致肠道微生物群的生态失调。很少有研究涉及抗生素对微生物组和宿主免疫的纵向影响。材料与方法本研究采用16S rRNA基因测序法评价氟喹诺酮类（左氧氟沙星）和β-内酰胺类抗生素（美罗培南、头孢哌酮/舒巴坦、氨曲南）对小鼠肠道菌群的短期影响。采用圆盘扩散法检测长双歧杆菌、乳酸乳杆菌、屎肠球菌和丁酸梭菌对抗菌药物的敏感性。结果显示，4天的抗生素暴露显著降低了肠道细菌的α和β多样性，增加了血清炎症因子，这些变化在停药后持续很长时间，并没有恢复到治疗前的水平。然而，停止治疗后，细菌群落组成趋于恢复到治疗前的水平。所检测的益生菌菌株对氨曲南耐药，但对美罗培南和头孢哌酮舒巴坦敏感。结论短期抗生素治疗可使肠道菌群发生明显变化，并有恢复趋势。抗生素对肠道微生物群落和益生菌菌株的影响不同。本研究为益生菌和抗生素的同时使用提供了指导，结果强调了负责任地使用广谱抗生素以防止本地微生物群的长期破坏的重要性。
Background Antibiotics play an important role in the treatment of infectious diseases. However, the overuse of antibiotics increases the spread of drug-resistant bacteria and causes dysbiosis of the intestinal microbiota. Few studies have addressed the longitudinal effects of antibiotics on the microbiome and host immunity. Materials and Methods In this study, the short-term effect of fluoroquinolone (levofloxacin) and β-lactam antibiotics (meropenem, cefoperazone/sulbactam, and aztreonam) on the gut microbiota of mice was evaluated by 16S rRNA gene sequencing. The susceptibility of Bifidobacterium longum, Lactobacillus lactis, Enterococcus faecium, and Clostridium butyricum to these antimicrobial agents was assessed using the disc diffusion method. Results Our results showed that 4-day antibiotic exposure significantly reduced the alpha and beta diversity of gut bacteria and increased serum inflammatory cytokines, and these changes persisted long after antibiotic withdrawal and did not return to pre-treatment levels. Nonetheless, the bacterial community composition tended to return to pre-treatment levels after discontinuing treatment. The tested probiotic strains were resistant to aztreonam but were sensitive to meropenem and cefoperazone/sulbactam. Conclusion Short-term antibiotic treatment led to significant changes in the intestinal flora with a tendency to recover. The antibiotics had different effects on the intestinal microbial community and probiotic strains. This study provides guidance for the concomitant use of probiotics and antibiotics, and the results emphasize the importance of using broad-spectrum antibiotics responsibly to prevent the long-term disruption of the native microbiota.