Goats' milk as a natural source of lactose-derived oligosaccharides: Isolation by membrane technology
Goats' milk as a natural source of lactose-derived oligosaccharides: Isolation by membrane technology
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DOI：
10.1016/j.idairyj.2005.02.003
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发表时间：
2006-02-01
影响因子：
3.1
通讯作者：
Kunz, C
中科院分区：
文献类型：
--
作者：
Martinez-Ferez, A;Rudloff, S;Kunz, C
人乳低聚糖被认为是有益于婴儿的益生元和抗感染特性。然而，到目前为止，没有来自农场动物的奶被认为是用于人类营养的乳糖衍生低聚糖的良好天然来源。在本研究中，对成熟山羊乳中的中性和唾液酸化乳糖衍生低聚糖进行了表征和定量，并与绵羊乳、牛乳和人乳进行了比较。采用高pH阴离子交换色谱-脉冲安培检测法（HPAEC-PAD）进行定量，采用快原子轰击质谱（FAB-MS）进行表征。与牛乳和绵羊乳相比，山羊乳中酸性和中性低聚糖含量高，种类多。此外，在山羊乳中鉴定了15种新的寡糖结构。采用两级切向超滤-纳滤工艺分离羊奶低聚糖。管状陶瓷膜的截留分子量分别为50和1 kDa。获得了含有超过80%的原始低聚糖含量的几乎不含乳糖和盐的产品。(c)2005爱思唯尔有限公司保留所有权利。
Human milk oligosaccharides are thought to be beneficial for the infant with regard to their prebiotic and anti-infective properties. However, so far no milk from farm animals has been considered to be a good natural source of lactose-derived oligosaccharides for human nutrition. In this study, the characterization and quantitation of neutral and sialylated lactose-derived oligosaccharides in mature caprine milk was performed and compared to ovine, bovine and human milk. The quantification was carried out using high-pH anion-exchange chromatography with pulsed amperometric detection (HPAEC-PAD), and the characterization was performed by fast atom bombardment mass spectrometry (FAB-MS). A large amount and variety of acidic and neutral oligosaccharides were found in goats' milk when compared with cow and sheep milk. In addition, 15 new oligosaccharide structures were identified in caprine milk. In order to isolate the goats' milk oligosaccharide fraction, a two-stage tangential ultrafiltration-nanofiltration process was selected. Tubular ceramic membranes with molecular mass cut-offs of 50 and 1 kDa, respectively, were employed. A virtually lactose and salts-free product containing more than 80% of the original oligosaccharide content was obtained. (c) 2005 Elsevier Ltd. All rights reserved.