[A new quantitative assay of heat shock protein 70 by based on an enzyme-linked immunosorbent assay method].
[A new quantitative assay of heat shock protein 70 by based on an enzyme-linked immunosorbent assay method].
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基于酶联免疫吸附法的热休克蛋白70定量检测新方法[J].
DOI：
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发表时间：
1996
期刊：
影响因子：
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通讯作者：
T. Oguri
中科院分区：
文献类型：
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作者：
A. Kikuchi;Y. Itoh;K. Seki;M. Morita;M. Kobayashi;M. Aihara;T. Oguri
热休克蛋白70(HSP70)是一种重要的应激蛋白。通过免疫印迹或HSP70的mRNA检测到HSP70的表达。我们的目的是建立一种简便的HSP70定量检测方法，并利用临床标本、细胞、组织等。我们研究了一种新的基于酶联免疫吸附试验(ELISA)的HSP70定量检测方法。该方法标准曲线重现性好，重现性好(CV：7.1，6.8%)，回收率(93~116%)。用该方法测定了热应激条件下人脐静脉内皮细胞(HUVEC)中HSP70的表达。HUVEC中的HSP70随着加热温度的升高而增加。结论：即使在细胞和组织中，HSP70的含量也是可能的。
Heat Shock Protein 70 (HSP70) was very important stress protein. HSP70 had been detected by immunoblotting or mRNA of HSP70. Our purpose is to develop an easy quantitative assay of HSP70 and utilize clinical samples, cells, tissues etc. We examined a new quantitative assay of HSP70 that was based on an enzyme-linked immunosorbent assay (ELISA). This quantitative assay showed reproducible standard curve, high reproducibility (CV : 7.1, 6.8%) and recovery (93 approximately 116%). The determination of HSP70 in human umbilical vein endothelial cells (HUVEC) by heat stress was assayed using this method. HSP70 in HUVEC increased according to the heat temperature. It is concluded that the quantity of HSP70 might be possible even in cells and tissues by this method.