PlasMatch
PlasMatch
复制标题
普拉斯匹配
DOI：
10.1145/2808719.2811570
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发表时间：
2015
期刊：
影响因子：
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通讯作者：
Goel S
中科院分区：
文献类型：
--
作者：
Goel S
当克隆基因以在目标生物中表达时，必须谨慎地部署基因工程工具库，以使其体外程序正常工作，例如发现哪些限制性内切酶可用于仅在所需位置创建子串和“粘端”。当克隆到细菌中时，为了避免不必要的相互作用，决定正确的质粒用于将新的生物回路转移到宿主中也可能是具有挑战性的。虽然生物信息学的进步已经产生了巨大的克隆序列和质粒数据库，但将这些数据库与特定的实验环境相匹配是费力且容易出错的。在这里，我们提供了易于使用的软件工具，可以参考标准和个性化数据库，以便为湿实验室工作人员找到正确的生物载体和工具选择。
When cloning genes to express them in target organisms, care must be taken in deploying the arsenal of genetic engineering tools to allow thein vitroprocedures to work correctly, such as finding which restriction enzymes can be used to create substrings and 'sticky ends' at only the desired locations. When cloning into bacteria, deciding on the right plasmid to be used to transfer new biocircuits into the host can also be challenging in order to avoid undesired interactions. While progress in Bioinformatics has resulted in huge databases of cloning sequences and plasmids, matching these to specific experimental settings is laborious and error-prone. Here we present easy to use software tools that consult standard and personalized databases in order to find the correct choices of biological vectors and tools for the wet-lab worker.