FGF19‐FGFR4 signaling elaborates lens induction with the FGF8‐L‐Maf cascade in the chick embryo
FGF19‐FGFR4 signaling elaborates lens induction with the FGF8‐L‐Maf cascade in the chick embryo
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DOI：
10.1111/j.1440-169x.2005.00795.x
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发表时间：
2005-05
期刊：
影响因子：
4.6
通讯作者：
Hitomi Kurose;M. Okamoto;M. Shimizu;T. Bito;C. Marcelle;S. Noji;H. Ohuchi
中科院分区：
文献类型：
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作者：
Hitomi Kurose;M. Okamoto;M. Shimizu;T. Bito;C. Marcelle;S. Noji;H. Ohuchi
已知成纤维细胞生长因子（FGF）家族参与脊椎动物眼睛发育。然而，个别FGF成员在眼睛发育过程中的独特作用在很大程度上仍然难以捉摸。在这里，我们展示了鸡透镜发育中Fgf 19的详细表达模式。Fgf 19表达起始于前脑，然后局限于邻近未来透镜基板的视泡的远端部分，其中表达FGF受体4（Fgfr 4），FGF 19的受体。Fgf 8是L-Maf的正调节因子，在视泡的一部分中表达。为了研究FGF 19信号在早期眼发育中的作用，在假定的透镜外胚层附近错误表达FGF 19;然而，未观察到透镜标记基因的表达改变。相反，FGFR 4的分泌形式被错误表达以抑制FGF 19信号，导致L-Maf表达的诱导。为了进一步确定L-Maf和Fgf 19之间的关系，进行了L-Maf错误表达，导致Hamburger和汉密尔顿12/13期异位诱导Fgf 19表达。此外，除了L-Maf之外，Fgf 8的错误表达还诱导Fgf 19的表达。这些结果表明，FGF 19-FGFR 4信号传导与FGF 8信号传导和L-Maf转录系统协作在早期透镜发育中起作用。
The fibroblast growth factor (FGF) family is known to be involved in vertebrate eye development. However, distinct roles of individual FGF members during eye development remain largely elusive. Here, we show a detailed expression pattern of Fgf19 in chick lens development. Fgf19 expression initiated in the forebrain, and then became restricted to the distal portion of the optic vesicle abutting the future lens placode, where FGF receptor 4 (Fgfr4), a receptor for FGF19, was expressed. Fgf8, a positive regulator for L‐Maf, was expressed in a portion of the optic vesicle. To examine the role of FGF19 signaling during early eye development, Fgf19 was misexpressed near the presumptive lens ectoderm; however, no alteration in the expression of lens marker genes was observed. Conversely, a secreted form of FGFR4 was misexpressed to inhibit an FGF19 signal, resulting in the induction of L‐Maf expression. To further define the relationship between L‐Maf and Fgf19, L‐Maf misexpression was performed, resulting in ectopic induction of Fgf19 expression by Hamburger and Hamilton's stage 12/13. Furthermore, misexpression of Fgf8 induced Fgf19 expression in addition to L‐Maf. These results suggest that FGF19‐FGFR4 signaling plays a role in early lens development in collaboration with FGF8 signaling and L‐Maf transcriptional system.