The Arabidopsis ref2 mutant is defective in the gene encoding CYP83A1 and shows both phenylpropanoid and glucosinolate phenotypes
The Arabidopsis ref2 mutant is defective in the gene encoding CYP83A1 and shows both phenylpropanoid and glucosinolate phenotypes
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DOI：
10.1105/tpc.006544
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发表时间：
2003-01-01
期刊：
影响因子：
11.6
通讯作者：
Chapple, C
中科院分区：
文献类型：
--
作者：
Hemm, MR;Ruegger, MO;Chapple, C
在筛选在UV光下具有改变的荧光的植物中鉴定拟南芥ref2突变体。ref2突变体的表征表明，它们含有降低水平的一些苯丙素途径衍生的产品：芥子酰苹果酸在叶子中，芥子酰胆碱在种子中，和甜菜碱木质素在茎。令人惊讶的是，REF 2基因座的定位克隆显示，它编码CYP83A1，一种与CYP83B1高度相似的细胞色素P450，一种参与芥子油苷生物合成的酶。进一步研究发现，ref2突变体叶片中所有脂肪族硫代葡萄糖苷水平降低，吲哚衍生的硫代葡萄糖苷水平升高。这些结果表明，CYP83A1参与短链和长链脂肪族硫代葡萄糖苷的生物合成，并建议硫代葡萄糖苷生物合成之间的一种新的代谢联系，一种仅在植物中发现的二级生物合成途径，在所有植物中发现的苯丙烷代谢途径，被认为是陆生植物物种生存所必需的。
The Arabidopsis ref2 mutant was identified in a screen for plants having altered fluorescence under UV light. Characterization of the ref2 mutants showed that they contained reduced levels of a number of phenylpropanoid pathway-derived products: sinapoylmalate in leaves, sinapoylcholine in seeds, and syringyl lignin in stems. Surprisingly, positional cloning of the REF2 locus revealed that it encodes CYP83A1, a cytochrome P450 sharing a high degree of similarity to CYP83B1, an enzyme involved in glucosinolate biosynthesis. Upon further investigation, ref2 mutants were found to have reduced levels of all aliphatic glucosinolates and increased levels of indole-derived glucosinolates in their leaves. These results show that CYP83A1 is involved in the blosynthesis of both short-chain and long-chain aliphatic glucosinolates and suggest a novel metabolic link between glucosinolate biosynthesis, a secondary biosynthetic pathway found only in plants in the order Capparales, and phenylpropanoid metabolism, a pathway found in all plants and considered essential to the survival of terrestrial plant species.