The integration characteristics of the exogenous growth hormone gene in a transgenic common carp (Cyprinus carpio L.) with fast-growth performance
The integration characteristics of the exogenous growth hormone gene in a transgenic common carp (Cyprinus carpio L.) with fast-growth performance
复制标题
外源生长激素基因在快生转基因鲤鱼中的整合特性
DOI：
10.1007/s11434-015-0893-x
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发表时间：
2015-10-01
期刊：
影响因子：
18.9
通讯作者：
Hu, Wei
中科院分区：
文献类型：
--
作者：
Chen, Ji;Luo, Qing;Hu, Wei
外源基因在转基因动物中的遗传稳定性和表达效率与整合位点和拷贝数密切相关。在本实验室，通过转基因操作和随后的测交，我们建立了一个“全鱼”生长激素（GH）转基因鲤鱼家族，表现出快速生长。本研究采用基因组步移、实时荧光定量PCR和荧光原位杂交技术，对外源草鱼GH基因在转基因鲤中的整合特征进行了研究。外源GH基因以两个完整和一个不完整串联重复序列的形式整合到染色体对末端附近的AT富集区。我们推测，外源GH基因的高效表达可能是由于基因组中的低拷贝数和富含AT的整合位点。
The genetic stability and expression efficiency of exogenous genes in transgenic animals are closely related to integration site and copy number. In our laboratory, by transgenic manipulation and subsequent test crosses, we established an "all-fish" growth hormone (GH) transgenic common carp family that exhibits fast growth. In this present study, genome walking, real-time quantitative polymerase chain reaction, and fluorescence in situ hybridization techniques were applied to identify the integration characteristics of the exogenous grass carp GH gene in the transgenic common carp. The exogenous GH genes, in the form of two complete and one incomplete tandem repeats, were found to have integrated into an AT-rich region near the end of a chromosome pair. We hypothesize that the high efficiency of exogenous GH gene expression might be due to the low copy number in the genome and the AT-rich integration site.