NrnA is a 5'-3' exonuclease that processes short RNA substrates in vivo and in vitro.
NrnA is a 5'-3' exonuclease that processes short RNA substrates in vivo and in vitro.
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DOI：
10.1093/nar/gkac1091
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发表时间：
2022-11-28
影响因子：
14.9
通讯作者：
Winkler, Wade C.
中科院分区：
文献类型：
--
作者：
Weiss, Cordelia A.;Myers, Tanner M.;Wu, Chih Hao;Jenkins, Conor;Sondermann, Holger;Lee, Vincent T.;Winkler, Wade C.
细菌rna酶处理rna直到只剩下短的寡聚物（2-5个核苷酸），然后由一个或多个专门的酶处理直到只剩下核苷单磷酸。寡核糖核酸酶（Orn）是发挥这种作用的一种重要酶。然而，许多细菌不编码Orn，而是编码纳米rna酶A （NrnA）。然而，NrnA蛋白的催化机制、细胞作用和生理相关底物尚未完全确定。在此，我们使用了一套常见的反应试验来直接比较枯草芽孢杆菌、粪肠球菌、化脓性链球菌和结核分枝杆菌的rna样蛋白对底物的偏好。虽然结核分枝杆菌蛋白特异性地切割环二磷酸腺苷，但枯草芽孢杆菌、粪芽孢杆菌和化脓性链球菌的rna样蛋白一致地对长度在2-4个核苷酸之间的短rna表现出惊人的偏好，这些短rna都是从它们的5 '端开始加工的。相应地，枯草芽孢杆菌nrnA的缺失导致细胞提取物中长度在2到4个核苷酸之间的rna的积累。综上所述，这些数据表明，许多厚壁菌门的rna样蛋白很可能类似枯草芽孢杆菌的NrnA，作为处理长度在2到4个核苷酸之间的rna的管家酶。
Bacterial RNases process RNAs until only short oligomers (2–5 nucleotides) remain, which are then processed by one or more specialized enzymes until only nucleoside monophosphates remain. Oligoribonuclease (Orn) is an essential enzyme that acts in this capacity. However, many bacteria do not encode for Orn and instead encode for NanoRNase A (NrnA). Yet, the catalytic mechanism, cellular roles and physiologically relevant substrates have not been fully resolved for NrnA proteins. We herein utilized a common set of reaction assays to directly compare substrate preferences exhibited by NrnA-like proteins from Bacillus subtilis, Enterococcus faecalis, Streptococcus pyogenes and Mycobacterium tuberculosis. While the M. tuberculosis protein specifically cleaved cyclic di-adenosine monophosphate, the B. subtilis, E. faecalis and S. pyogenes NrnA-like proteins uniformly exhibited striking preference for short RNAs between 2–4 nucleotides in length, all of which were processed from their 5′ terminus. Correspondingly, deletion of B. subtilis nrnA led to accumulation of RNAs between 2 and 4 nucleotides in length in cellular extracts. Together, these data suggest that many Firmicutes NrnA-like proteins are likely to resemble B. subtilis NrnA to act as a housekeeping enzyme for processing of RNAs between 2 and 4 nucleotides in length.
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影响因子：
11.1
作者：
Hui MP;Foley PL;Belasco JG
通讯作者：
Belasco JG
影响因子：
14.9
作者：
Fang M;Zeisberg WM;Condon C;Ogryzko V;Danchin A;Mechold U
通讯作者：
Mechold U
影响因子：
3.2
作者：
Gall, Aaron R.;Hsueh, Brian Y.;Huynh, TuAnh N.
通讯作者：
Huynh, TuAnh N.
影响因子：
48
作者：
Gibson, Daniel G.;Young, Lei;Smith, Hamilton O.
通讯作者：
Smith, Hamilton O.
影响因子：
5.7
作者：
Drexler, David Jan;Mueller, Martina;Witte, Gregor
通讯作者：
Witte, Gregor